MIQE 荧光定量zcl.ppt

* How to design your runs? 4 X 96-well plates (runs) 4 different target genes (A - H) 30 different samples (1 -16) Two strategies to design these runs: Gene maximization and Sample maximization No.1 * N0. 2 * N0. 3 * Inter-run calibration * Whats the difference of expression profile between IRC and non-IRC ? IRC non-IRC * Tips and tricks 微滴式数字 PCR – 第三代PCR 常规PCR 定性 1st 2nd 3rd 定量PCR 相对定量 微滴式数字 PCR 绝对定量 微滴分析仪 微滴发生器 The future of PCR is here, and it’s digital QX100TM微滴式数字PCR系统 微滴式数字PCR流程 将20ul含有样品的PCR预混液制备成20,000个微滴 进行emulsion PCR PCR扩增完成后，对每个样品中的微滴逐个检测，有荧光信号的微滴为阳性微滴；反之为阴性微滴 根据记录到