黄瓜对南美斑潜蝇为害生理生化响应-农业昆虫与害虫防治专业论文 word格式.docxVIP

Physiological and Biochemical Responses of Cucumber Against Infestation by Liriomyza Huidobrensis (Diptera: Agromyzidae)AbstractThe physiogical and biochemical responses and mechanism of cucumber after infested by Liriomyza huidobrensis（Blanchard） larvae were studied. The changes of phenylalanine ammonia-lyase(PAL), polyphenol oxidase(PPO), peroxidase(POD),superoxide dismutase(SOD), protease inhibitors(PIs), soluble sugar, soluble protein, chlorophyll, tannin, flavone, cucurbitacin B(CuB), salicylic acid(SA) and jasmonic acid(JA) after Liriomyza huidobrensis larvae feeding continuously in cucumber leaves were tested. The main results showed that:The contents of soluble sugar, soluble protein and chlorophyll in cucumber leaves decreased with the increase of damage by the larvae. The minimum contents of soluble sugar, soluble protein and chlorophyll appeared on the 7th, 5th and 7th day, respectively, in the damaged-lightly leaves, and on the 5th, 5th and 7th day, respectively, in the damaged-heavily leaves.The contents of tannin, flavone and CuB in cucumber leaves increased with the increase of damage by the larvae. The maximum contents of tannin, flavone and CuB appeared on the 7th, 5th and 3rd day, respectively, in the damaged-lightly leaves, and on the 5th, 3rd and 5th day, respectively, in the damaged-heavily leaves. They were on the 5th, 3rd and 3rd day, respectively, in the lightly-systemic control leaves, and on the 3rd, 3rd and 1st day, respectively, in the heavily-systemic control leaves. The results suggest that the influences of the larval infestation on the contents of tannin and flavone were systemic, and it was systemic on the content of CuB in cucumber leaves in the early stages of the infestion.The activities of PAL, PPO, POD and SOD in cucumber leaves which had been feeding by the larvae rose significantly. The magnitude of activity rising of PAL, PPO, POD and SOD increased with the increase of damaged levels, i.e. damaged heavily lightly sy