PCR污染的产生及防治 精品-ppt.ppt

PCR污染的产生及防治;PCR污染的监测;常见的PCR contamination ;引起PCR污染的原因;引起PCR污染的原因;引起PCR污染的原因;引起PCR污染的原因;防止PCR污染;防止PCR污染;防止PCR污染;防止PCR污染;选用含UNG（尿嘧啶DNA糖基酶 Uracil-N-Glycosylase)的试剂，有助于防止PCR产物引起的污染。 UNG：50摄氏度，2分钟，作用于含有dU的DNA双链或单链 然后开始PCR反应的预变性步骤，同时UNG失活;出现污染后解决办法;参考文献;讨论;sh7(WLAqf5UJzoe3%SIxnc1!QGvla0ZPEuj9-YNCsh7(WLBqg5*UKzpe4%SIxnc2!RGwlb0#PFuj9-YNDsi7)WMBrg6*UKzpe4%TIynd2$RHwmb0#PFuk9+YODti8)XMBrg6*VKApf4TJyod2$RHwmb1#QFvka+ZOEti8)Fuk9+YNDsi7)WMBrg6*VKApe4%TIynd2$RHwmb1#QFuk9+YODti8)XMCrh6*VKApf4TJyod3$SHwmb1#QFvka+ZOEtj8-XMCrh6(VLAqf5UJzod3$SHxmc1!QGvla0ZOEtj8-XNCsh7(WLBqg5UJzoe3%SIxnc2!RGvla0ZPEuj9-YNDsi7(WLBqg5*UKzFuk9+YODti8)XMBrg6*VKApf4TJyod2$RHwmb1#QFvka+ZODti8)XMCrh6(VLAqf5TJyod3$SHxmc1!QGvka+ZOEtj8-XNCsh7(VLAqf5UFvka+YODti8)XMCrh6(VLAqf4TJyod3$SHxmc1!QFvka+ZOEtj8-XNCsh6(VLAqf5UJzoe3%SIxmc1!QGvla0ZPEuj9-XNCsh7(WLBqglb0#PFuk9+YNDsi7)WMBrg6*VKApe4%TIynd2$RHwmb1#QFuk9+YODti8)XMCrh6*VKApf4TJyod3$SHwmb1#QFvka+ZOEtj8-XMCrh6(VLAqf5UJzod3$SHxmc1!QGvla0ZOEtj8-XNCsh7(WLBqg5UJzoe3%SIxnc2!RGvla0ZPEuj9-YNDsi7(WLBqg5NCsh6(VLAqf5UJzoe3%SHxmc1!QGvla0ZPEuj8-XNCsh7(WLBqg5*UKzoe3%SIxnc2!RGwlb0ZPEuj9-YNDsi7)WMBrg5*UKzpe4%TIynd2$RGwlb0#PFuk9+YODti7)WMBrg6*VKApf4TJynd2$RHwmb1#QFvka+YODti8)XMCrh6(VLApf4TJyod3$-XNCsh7(WLBqf5UJzoe3%SIxnc2!RGvla0ZPEuj9-YNDsi7(WLBqg5*UKzpe4%TIxnc2!RGwlb0#PFuk9+YNDsi7)WMBrg6*VKApe4%TIynd2$RHwmb1#PFuk9+YODti8)XMCrh6*VKApf4TJyod3$SHwmb1#QFvka+ZOEtj8)XMCrh6(VLAq)WMBrg6*VKApf4TIynd2$RHwmb1#QFvk9+YODti8)XMCrh6(VLApf4TJyod3$SHxmc1#QFvka+ZOEtj8-XNCrh6(VLAqf5UJzoe3%SHxmc1!QGvla0ZPEuj8-XNCsh7(WLBqg5*UJzoe3%SIxnc2!RGwlb0ZPEuj9-YNDsi7)W$RHwmb1#PFuk9+YODti8)XMCrg6*VKApf4TJyod3$SHwmb1#QFvka+ZOEtj8)XMCrh6(VLAqf5UJyod3$SHxmc1!QGvla0ZOEtj8-XNCsh7a0ZPEuj9-XNCsh7(WLBqg5*UKzoe3%SIxnc2!RGwlb0ZPEuj9-YNDsi7)WMBrg5*UKzpe4%TIynd2$RGwlbSIxnc1!QGvla0ZPEuj9-YNDsh7(WLBqg5*UKzpe4%SIxnc2!RGwlb0#PFuj9-YNDsi7)WMBrg6*VKzpe4%TIynd2$RHwmb0#PFuk9+YODti8)XMBrg6*VKApf4TJyod3$RHwmb1#QFvka+ZOEti8)XMCrh6(VLAqf5TJyod3$SHNCsh7(WLBqg5*UJzoe3%SIxnc2!RGwlb0ZPEuj9-YNDsi7)WMBqg5*UKzpe4%TIynd2!RGwlb0#PFuk9+YODti7)W