Basic gas chromatography - Fakultas Farmasi Universitas Andalas基本的气相色谱- fakultas farmasi安达拉斯大学精品.pdf

Basic gas chromatography prof. aza Department of Pharmacy, Andalas University STIFI Perintis, Padang STIFAR, Pekan Baru STIFI Bhakti Pertiwi, Palembang prof. aza Definition • The basis for gas chromatographic separation is the distribution of a sample between two phases. One of these phases is stationary bed of large surface area, and the other phase is gas which percolates through the stationary bed. • If the stationary phase is a solid, we speak of Gas- Solid Chromatography. This depends up on the adsorptive properties of the column packing to separate samples, primarily gases. • If the stationary phase is a liquid, we speak of Gas liquid Chromatography (GLC). The liquid is spread as a thin film over an inert solid and the basis for separation is the partitioning of the sample in and out of this liquid film. prof. aza Schematic drawing of Gas Chromatographic System prof. aza GC/MS • Gas Chromatography/Mass Spectrometry (GC/MS) is a combination of two instrumental techniques, gas chromatography and mass spectrometry. The gas chromatograph is used to separate a mixture into component parts and deliver them to the mass