Engineering Deinococcus radiodurans for metal remediation ：耐辐射球菌金属修复工程[精品].ppt

The Mercury Crisis * 4.2-kb mer operon of pBD724 encodes six proteins: MerR: activation/repression of the mer operon MerT: mercuric ion transport protein MerP: periplasmic mercuric ion binding protein MerC: transmembrane protein MerA: mercuric reductase MerD: putative secondary regulatory protein OP: operator/ promoter sequence * G-S-S-G 2 G-SH NADPH + H+ NADP+ FAD Glutathione reductase Glutathione Redox: * * * In this paper, the authors had cloned two different functional loci from bacteria into Deinococcus radiodurans for the purpose of bioremediation. What are those two functional loci? Please specify their names and main functions respectively. Engineering Deinococcus radiodurans formetal remediation in radioactive mixedwaste environments William Chen Keng Teo * Overview Background Deinococcus radiodurans Engineering Deinococcus radiodurans Experimental results and analysis Discussion Conclusions Future Development * Background In U.S. alone, 1000 waste sites with radiation level ? 10 mCi/L 7.5 x107 m3 soil 2 x1012 dm3 ground water contaminated by 3 x106 m3 leaking waste Clean-up cost $265 billion Potential targets for less expensive and more effective bioremediation methods * What is bioremediation? Definition: Use of biological mechanisms to destroy, transform or immobilize environmental contaminants to protect potential sensitive receptors Applications: Agricultural chemicals, gasoline contamination radioactive wastes….etc. Examples: 1) Xenobiotics by Pseudomonas sp. 2) n-alkane metabolism by Desulfobacterium cetonicum Pseudomonas sp. * Denococcus radiodurans Physiology: Gram(+), Non-pathogenic Red-pigmented Radiation-resistant: UV Ionic Selective solvent tolerant Non-motile Soil bacterium * Denococcus radiodurans R1 strain genome: 2 chromosomes (2.65 Mbp 412 kbp), with 4-10 genome equivalents/copies in growing cells 1 megaplasmid (177 kbp) 1 s