甘肃麦积山景区3种典型裸子植物土壤微生物群落结构-微生物学报.PDF

微生物学通报 Sep. 20, 2016, 43(9): 1939−1944 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.150797 研究报告 甘肃省麦积山景区3 种典型裸子植物土壤微生物 群落结构分析 * 陈利云 王弋博 李三相 汪之波 ( 741000) 摘 要：【目的】探明不同种类的植物对其根际土壤微生物数量分布与群落结构的影响。【方法】 将微生物计数法与磷脂脂肪酸(PLFA)法相结合，分析比较麦积山景区3 种典型裸子植物根际土 壤微生物的数量分布和PLFA 种类、含量及主成分结构。【结果】3 种植物根际土壤微生物数量 均以细菌最多，真菌最少；总PLFA 含量以红豆杉[Taxus chinensis (Pilg) Rehder.]最高、种类最 多，日本落叶松[Larix kaempferi (Lamb) Carriere.]最低，红豆杉与云杉(Picea asperata Mast) PLFA 主要成分相似度高于日本落叶松；外来种日本落叶松无论微生物数量，还是种类以及PLFA 结 构组成与红豆杉、云杉均有较大的差异，多样性显著下降。【结论】与土著裸子植物相比，外 来种日本落叶松能明显改变根际土壤微生物数量分布与群落结构。 关键词：麦积山景区，裸子植物，根际土壤，微生物群落，磷脂脂肪酸 Microbial community structure of three typical gymnosperms soil in scenic area of Maijishan, Gansu Province * CHEN Li-Yun WANG Yi-Bo LI San-Xiang WANG Zhi-Bo (School of Bioengineering and Technology, Tianshui Normal University, Key Laboratory of Agriculture Microbiology in Gansu Province, Tianshui, Gansu 741000, China) Abstract: [Objective] In order to identify the microbial community structure of the different gymnosperm types. [Methods] Microbial culture and PLFA (Phospholipid fatty-acid analysis) methods were employed to study the microbe quantity, PLFAs types and content of three typical gymnosperms soil in scenic area of Maijishan, Gansu Province. [Results] There existed distinct difference on composition and structure of soil microbial. Microbial number was highest in bacteria