小麦矮腥黑粉菌的鉴定及检测方法.doc

小麦矮腥黑粉菌的鉴定及检测方法 ? 128? 舷扬铱第33卷第6期(2007) PLANTPROTECTIONVo1.33No.6(2007) r3O]ROBERTSCA,DIETZGENRG,HEELANLA,eta1.Re al-timeRT-PCRfluorescentdetectionofTomatospottedwilt v/rus[J].JVirolMethods,2000,88(1):l一8. [31]EUNAJ,SEOHM,WONGS.Simultaneousquantification oftwoorchidvirusesbytheTaqManreal—timeRT_PCR[J].J VirolMethods,2000,87:151—160. [32]孙淑斌,李宝珍,胡江,等.水稻低丰度表达基因0sAMT1;3 实时荧光定量PCR方法的建立及其应用[J].中国水稻科学, 2006,20(1):8—12. [331NICOTN,HAUSMANJF,HOFFMANNL,eta1.House keepinggeneselectionforreal—timeRTPCRnormalizationin potatoduringbioticandabioticstress[J].JournalofExperi— mentalBotany,2005,56:2907—2914. r34]VOLKOVRA,PANCHUKII,SCHOFFIFHeat—stress— dependencyanddevelopmentalmodulationofgeneexpression: thepotentialofhousekeepinggenesasinternalstandardsin mRNAexpressionprofilingusingreal-timeRT-PCR[J].Jour— nalofExperimentalBotany,2003,54:2343—2349. [35]LISS13.Improvedquantitativereal—timeRT-PCRforexpres— sionprofilingofindividualceils[J].NucleicAcidsResearch, 2002,30(17):e89. [36:OHDANT,FRANCIS(;()PBJr,SAWADAT,eta1.Ex— pressionprofilingofgenesinvolvedinstarchsynthesisinsink andsourceorgansofrice[J].JournalofExperimentalBotany, 2005,56:3229～3244. [37吴茂森,田峰,齐放军,等.水稻与白叶枯病菌互作的基因表 达谱分析与差异性表达基因的识别[J]_中国农业科学,2007, 40(2):277—282. 小麦矮腥黑粉菌的鉴定及检测方法 董微,陈万权,刘太国 (中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100094) 摘要由TilletiacontrozaersaKiihn(TCK)引起的小麦矮腥黑穗病是重要的国际检疫性病害,也是我国对外的A 类检疫对象.本文就该病原菌的形态学和生物学鉴定,分子检测方法进行了综述.展望了分子检测技术在病害检 疫中的应用前景. 关键词小麦矮腥黑粉菌;形态学;生物学;分子检测 中图分类号S435.12 IdentificationanddetectionmethodsofT/lletiacontroversnKfihn DongWei,ChenWanquan,LiuTaiguo (StateKeyLaboratoryforBiologyofPlantDiseaseandInsectPests,InstituteofPlantProtection, CAAS,Beijing100094,China) AbstractDwarfbuntofwheat,causedbyTilletiacontroversaKfihn(TCK),isanimportantinternationalquaran— finediseaseonwheatandthesameistrueforChina.Morphological,biologicalandmolecularbiologicalmethodsfor detectionofTCKweresystematicallyreviewedinthispaper.Prospectsformoleculardetection,diagnosisandmoni— toringofthisdiseasewerealsoputforward. KeyWO