id基因在多种肺癌细胞中的表达及意义-现代生物医学进展.pdfVIP

Progress in Modern Biomedicine 2006 Vol. 6 No. 2 36 Id 基因在多种肺癌细胞中的表达及意义 李晓莉 赵艳滨 孙立春 李乐静 ( 黑龙江 哈尔滨 150040) :研究Id 基因在肺癌和永生化支气管上 细胞中的表达, 探讨其在肺癌细胞中表达的意义: 利用半定量 RT- PCR 和Western blot 方法检测多种肺癌和永生化支气管上 细胞中Id1- Id4mRNA 和 Id1- Id4 蛋白的表达: A549NCI - H460NCI- H446S - MES- 1Anip973 中Id1- Id3mRNA 均高表达, Id1 相对表达较强;而 AGZY 和MP- 184 中未表达Id1- Id3mRNA; 腺癌细胞均表达了Id4mRNA, 而NCI- H446S - MES- 1 未表达Id4mRNAA549,NCI- H460,NCI- H446, S - MES- 1,Anip 973 中Id1, Id2, Id3 蛋白均高表达, A549, NCI- H446, Anip 973 中Id2 的表达高于 NCI- H460, S - MES- 1; A549, NCI- H460,Anip 973 有Id4 的高表达,NCI- H446,S - MES- 1无Id4 的表达,Id1- Id4在AGZY 和MP- 184 中均未表达:4 种Id 基因均作为癌基因在肺癌的发生发展中发挥作用,Id1,Id2,Id3 与肺癌细胞的恶性程度以及增殖和转移密切相关, Id4 可做为肺腺 癌的检测标志物 :肺癌; Id 基因;癌基因 The expression and significance of Id in different lung cancer cells 1 1 1 1 LI Xiao- li , ZHAO Yan- bin , SUN Li- chun , LI Le -j ing Department of Internal Me icine , the Thir Aff iliate Hospital, Harbin Me ical University, Harbin 150040, China) ABSTACT Objective To investigate the expression and significance of Id gene in lung cancer and immortalization bronchial epithelial cells. Methods The expressions of Id1- Id4 mRNA and their proteins were examined by semiquantitative RT- PCR and Western blot assay. Results Id1to Id3 mRNA were high expressed inA549, NCI- H460, NCI- H446, S - MES- 1and Anip973 cells but not inAGZY andMP - 184 cells. The expression of Id1was much higher than others. Id4 mRNA was expressed in adenocarcinoma cells but not in NCI- H446 and S - MES- 1 cells. The proteins of Id1to Id3 were high expressed in A549, NCI- H460, NCI- H446, S - MES- 1 and Anip973 cells. The expression of Id2 in A549, NCI- H446 and Anip973was higher than that in NCI- H460 and S - MES- 1. Id4 was high expressed in A549, NCI- H460 and