豆豉水提物对α-葡萄糖苷酶体外抑制活性影响-食品科学.pdfVIP

64 2010, Vol. 31, No. 17 食品科学 ※基础研究 α 1 2 陈 静 ，李里特 (1.北京物资学院物流学院，北京 101149 ；2. 中国农业大学食品科学与营养工程学院，北京 100083) 本研究收集全国各地的 37 种豆豉样品，测试其水提物对 α- 葡萄糖苷酶体外抑制活性。结果表明：37 种来源不同的豆豉样品水提物均对小鼠肠道内的 α- 葡萄糖苷酶呈现一定程度的抑制活性，其中 5#(L.Y) 、16# (W.N.H) 以及21#(M.W)豆豉水提物对 α- 葡萄糖苷酶呈现较高的抑制活性(P ＜0.05)；此外，通过对高活性豆豉的 蔗糖和葡萄糖含量检测，结果表明，尽管豆豉中含有微量的葡萄糖，但抑制干扰作用甚微。本研究结果预示 着豆豉中存在可以强烈抑制 α- 葡萄糖苷酶的活性成分，这种活性成分呈现较好的水溶性，可能是具有类似糖结 构的成分在起作用，有待于深入研究。 豆豉；水提物； α- 葡萄糖苷酶抑制；活性成分 in vitro Inhibitory Effect of Water Extract from Douchi on Alpha-glucosidase Activity 1 2 CHEN Jing ，LI Li-te (1. Logistics School, Beijing Wuzi University, Beijing 101149, China； 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) Abstract ：Thirty-seven Douchi samples collected from various regions of China were tested for the inhibitory effects of their water extracts on alpha-glucosidase in vitro. The water extracts from all these Douchi samples could inhibit mouse intestinal alpha-glucosidase to a certainty extent. In addition, samples NO.5 (L.Y), NO.16 (W.N.H) and NO.21 (M.W) showed stronger inhibitory effect against the enzyme (P ＜ 0.05). Moreover, microamounts of glucose existing in Douchi samples had less interrupting effect on the anti-hyperglycemic activity of Douchi water extract. This study suggests that there are some active components in Douchi, which can strongly inhibit alpha-glucosidase and present excellent water solubility. These components may have sacchride-like structures. However, this needs to be deeply investigated. Key words