不同强度仿生脑电场刺激对抑郁大鼠额叶单胺类递.docVIP

电刺激小脑顶核对抑郁大鼠额叶单胺类递质含量的影响 吴静 邓志宽a (第三军医大学附属新桥医院神经内科，重庆，400037) 提要： 目的 观察电刺激小脑顶核对抑郁大鼠额叶单胺类递质含量的影响，探讨电刺激小脑治疗抑郁症途径及机制。方法 建立Wistar大鼠抑郁症模型；模型大鼠在左侧小脑顶核（FN）或齿状核（DN）放置电极并进行电刺激；应用荧光分光光度法分别测定大鼠两侧额叶脑组织内五羟色胺（5-HT）、去甲肾上腺素（NE）和多巴胺（DA）含量。结果 与正常对照组相比，模型组大鼠双侧额叶内5-HT及NE含量显著下降（分别p0.001），而DA含量无明显改变。正常对照动物刺激FN和DN后，所观察的三种单胺类递质均无明显改变（分别p0.05）。模型动物FN刺激后左侧和右侧额叶NE及5-HT含量均有显著增高（分别p0.05，p0.001；p0.05，p0.01），而右侧增高更明显，双侧相比有显著差异（分别p0.05）。模型动物DN刺激后左侧和右侧额叶所观察的三种单胺类递质均无明显变化（分别p0.05）。结论 电刺激小脑顶核可显著增加抑郁大鼠额叶内NE及5-HT含量，提示对抑郁症可能具有治疗作用。 关键词：电刺激；小脑顶核；抑郁症；单胺类 Effect of electrical stimulating cerebellar fastigial nucleus on the contents of monoamines in the frontal lobe of rats with depression Wu Jing, Deng Zhi-kuan (Department of Neurology, the Second Affiliated Hospital, the Third Military Medical University, Chongqing, 400037,China) ABSTRACT: Objective To investigated the effect of electrical stimulating cerebellar fastigial nucleus on the contents of monoamines in the frontal lobe of rats with depression and explore the pathway and mechanism of electrical stimulating cerebella in treating depression. Methods Wistar rats were exposed to chronic stress circumstances to establish depression model of rats. After 24h, depressed rats were given electrical stimulating left cerebellar fastigial nucleus or left dentate nucleus for 1h and the contents of serotonin, noradrenalin and dopamine in frontal lobe were determined with fluorospectrophotometry. Results Compared with normal control group, the contents of serotonin and noradrenalin in bilateral frontal lobes of depression modle rats decreased obviously(p0.001,respectively). All three monoamines investigated had no significant change in normal rats with stimulating FN or DN. But in stimulating FN group with depression, the contents of serotonin and noradrenalin in right and left frontal lobes increased significantly (p0.05, p0.001; p0.05, p0.01 respectively), the increase in right lobe was more obvious than in left one (p0.05 respectively). In stimulating