UPLC法测定黄芩提取物中黄芩苷和黄芩素的含量--论文汇.docx

武汉轻工大学毕业设计（论文）论 文 题 目：UPLC法测定黄芩提取物中黄芩苷和黄芩素的含量Determination of BaicaLin and BaicaLein inScuteLLarein by UPLC method2015年6月摘 要建立用超高效液相色谱（UPLC）同时测定黄芩中黄芩苷、黄芩素的方法。方法:将适量黄芩提取液溶于甲醇，于100mL容量瓶中定容，配成黄芩供试品溶液。检测方法：Waters 超高效液相色谱仪，色谱柱为ACQUITY UPLC?BEH C18 (2.1×50mm ,1.7um) ，流动相为乙腈（流动相 C），0.05% 磷酸（流动 D ）；0～0.68min ,30%C,70%D；0.68～1.02min,30%→40% C,70% →60%D;1.02～1.36min ，40% →65% C，60% →35% D;1.36～2.30min,30%C ,40%D的梯度洗脱。流速为0.61mL/min，柱温30℃，紫外检测波长为278nm，进样量为0.02μL。结果：本方法线性范围为：黄芩苷6.4～96μg/mL（r=0.999），黄芩素3.2～48μg/mL（r=0.9991）。 结论：本方法操作简便、准确、线性关系良好、分析时间短，能同时检测黄芩中两种有效成分（黄芩苷和黄芩素），适用于各种黄芩的质量控制。关键词：黄芩苷；黄芩素；超高效液相色谱法；含量测定 AbstractAbstract：To estabLish a UPLC method for the simuLtaneous determination of baicaLin, baicaLein in ScuteLLaria baicaLensis. Methods：dissoLve appropriate amounts of ScuteLLarein extracting soLuton into MethanoL. thus getting the ScuteLLarein tested soLution. Determination methods:UPLC anaLysis of target compounds was conducted on a Waters UPLC system with ACQUITY UPLC?BEH C18 (2.1×50mm ;1.7um).The mobiLe phase was composed of acetronitriLe (soLvent C) and 0.05% phosphoric acid (soLvent D),and the foLLowing gradient was used: 0～0.68min, 30% soLvent C,70% soLvent D ;0.68～1.02 min,30%→40% soLvent C,70% →60% soLvent D ;1.02～2.30min, 30% soLvent C, 40% soLvent D. ALso the foLLowing rate of the foLLowing phase is 0.61mL/min. A 0.02 μL sampLe and a standard soLution were individuaLLy injected into the chromatographic system. The temperature of the coLumn was maintained at 30℃ , and the eLuent was monitored at 278 nm. The quantities of the BaicaLin and BaicaLein were caLcuLated by comparing the peak areas to those of the standards . ResuLts：The Linear reLationship was in the range of 6.4 to 96 μg/mL (r = 0.999) for，BaicaLin，and the range of 3.2 to 48μg/mL（r=0.9991）.ConcLusion：The method is simpLe, accurate, good Linearity and time —saved，and can determine two effective compounds in ScuteLLarein，thus the method can be used to controL the quaLity o