基质金属蛋白酶-23在退变颈椎间盘髓核组织中的表达及意义.pdfVIP

第42 卷 第2 期 兰州大学学报（医学版） Vol.42 No.2 2016 年4 月 Journal of Lanzhou University （Medical Sciences ） Apr. 2016 文章编号：1000-2812 (2016) 02-0068-05 基质金属蛋白酶-2 ，3 在退变颈椎间盘髓核 组织中的表达及意义 王 颢，王永刚，周海宇 兰州大学第二医院骨科，甘肃省骨关节疾病重点实验室，甘肃 兰州 730030 摘要：目的目的通过观察退变颈椎间盘髓核组织基质金属蛋白酶-2 （MMP-2 ）和MMP-3 表达的变化，探讨其在颈椎间盘 退变过程中的作用及意义。方法方法 55 例颈前路椎间盘摘除术的颈椎病患者髓核标本作为实验组，30 例颈椎间盘切除术的 颈椎外伤患者髓核标本作为对照组。将取出的标本固定后切片染色，采用免疫组化方法对标本内MMP-2 和MMP-3 染 色阳性的细胞进行定性和半定量分析，观察其表达变化。结果结果 实验组髓核细胞和软骨细胞的胞浆中MMP-2 和MMP-3 染色阳性率分别为78.18% 和70.91% ，明显高于对照组 （P 0.05 ）。结论结论 MMP-2 和MMP-3 在退变颈椎间盘中高表达， 二者可能在颈椎间盘退变中发挥了一定作用。 关键词：颈椎间盘退变；基质金属蛋白酶-2 ；基质金属蛋白酶-3 中图分类号：R446.5 文献标识码：A doi ：10.13885/j.issn. 1000-2812.2016.02.014 Expression and significance of matrix metalloproteinase-2, 3 in nucleus pulposus of degeneration cervical intervertebral disc Wang Hao, Wang Yong-gang, Zhou Hai-yu Department of Orthopedic, Key Laboratory of Osteoarticular Diseases of Gansu Province, Second Hospital of Lanzhou University, Lanzhou 730030 , China Abstract ：：Objective To study the expressions and significance of matrix metalloproteinase- 2 (MMP- 2) and matrix metalloproteinase- 3 (MMP- 3) in nucleus pulposus of degenerative cervical intervertebral disc. Methods 55 patients with cervical spondylosis underwent anterior cervical discectomy and taken as experi- mental group. While 30 patients with cervical spine trauma received cervical discectomy as control group. Ob- tained specimens were fixed, sectioned and stained, the expression levels of the MMP-2 and MMP-3 were de- termined qualitatively and semiquantitatively by immunohistochemical methods. Results In the experimental group, the positive rates of MMP-2 and MMP-3 staining in the cytoplasm of nucleus pulposus cells and chon- drocytes were 78.18% and 70.91%, respectively, which were significantly higher than those in control gro