小鼠x染色体性发育启动相关qtl的精细定位-生物化学与分子生物学专业论文.docx

小鼠X染色体性发育启动相关QTL的精细定位一IcM (物理距离z.6Mb)的区段内。对性发育启动调控机制的研究，有助于揭示性发育整个生理过程的相关调控机制，也有助于我们对个体发育过程以及相关神经内分泌系统的作用机理加深理解。本研究的结果为性发育启动基因进一步的的定位克隆研究打下了良好的基础。同时确证了x染色体(性染色体)存在相关基因，参与了性发育启动相关通路的调节。所有这些工作均有助于帮助我们理解性发育的整个生理过程和调控机制。关键词:性发育启动，特异性区段同类系，数量性状基因座，精细定位小鼠X染色体性发育启动相关 QTL的精细定位A DOM INANT X一LINKED QTL REG ULATINGPU BERTA L TIM INGINM ICE FO U N DBY FINEM A PPIN GA BST RA C TPuberty，often influeneedbybothenviromentalandgenetiefaetors，15 a eom Plieatedbiologie Proeess involvingsexualm aturationandgainingabilityof reProduetion.O nset of Puberty15 triggeredbyreactivationofthe hyPothalamie一Pituitary一gonadalaxis(HPGA)andm odulated by both genetie and environm entalfactors.Previou studiessuggested thatehromosomeXeould habor gene(s) regulating thePubertalonset.W e PreviouslymaPPed a QTL inthe age ofvaginaloPeningtoa一2.5eentimorgan(eM)interval(DXMit225一DXMitl40)ofmouse chromosomeX.Here，we narrowed the QTLto a 一leM(1.6Mb)intervalbymodifiedinterval一sPeeifieeongeniestrain(mISCS)analysis.Prev iousw ork in ourlab w as thatw e Perform ed a genom e一w idescanningforlinkageinreeiProcalcrossesbetw eentw ostrains，C3H/HeJ(C3H)and C57BL6/J(B6)，whieh differed significantly inthe Pubertaltim ing，VaginaloPening(VO)wasused to charaeterizePubertaltiminginfem alem ice(twoParentalstrains，F1andFZProgeny)Wasreeorded.Agenome一wide seareh was PerformedinPhenotyPieallyextrem e FZfem alem ieeout ofProgenyoftheFIintererossesto identify quantitative traitloei(QTLs)eontrolling thistrait.AQTLsignifieantlyassoeiatedwasmaPPedto一25cM(DxM itlo3一DXM itllg，LOD=3.86).The loeation ofthe significantQTL onChrXwasthenfine一maPPed toaregionof一2.scMbetweenrn arkerD XM it225 and D XM it】40.Inthisstudy，M alem ieeheldB 6genesintheD X M it225一D X Mitl40intervalw ereehosenbygenotyPingandeontintled to baekerossw ithC 3H forthree tim esto F7.The age atV O ，小鼠X染色体性发育启动相关盯L的精细定位firstcornifieation，and onsetofeyelicityofallfemale m ice (F7Progeny)wasreeorded ，and the antraldiam eteroffollielesfrom26一da