妊娠后期宫内生长限制对蒙古绵羊胎儿肝脏细胞凋亡及信号转导途径的影响-动物营养与饲料学专业论文.docx

EffectsofIntrauterineGrowthRestrictionDuringLatePregnancyon LiverCellApoptosisandSignalPathwayinMongoliaOvineFetusAbstractThispapermainlyinvestigatedtheeffectsofintrauterinegrowthrestrictionduring latepregnancyonlivercellapoptosisandsignalpathwayinMongoliaovinefetusandin ordertoprovidetheoreticalbasisandreferenceforanti-disaster,protectinglivestock, ewes’feedingandmanagementduringlatepregnancy,clinicalapplications.Thetechniquesofsynchronousestrusandartificialinseminationwereappliedon35 Mongolianewes,whichhadsimilarliveweight（mean52.38±2.03kg）.6eweswereslaughteredat90dofgestationandtheremaining29animalswereallocatedtothree differentgroups:RestrictedGroup1（RG1,0.175MJME·kgw-0.75·d-1,n=12）,Restricted Group2（RG2,0.33MJME·kgw-0.75·d-1,n=9）andControlGroup（CG,adlibitum,0.67 MJME·kgw-0.75·d-1,n=8）.At140dofgestation,6representativeewesineachgroupwereslaughtered.EffectsofmaternalundernutritionduringlatepregnancyonMongoliaewesand theirfetusWiththenutrientssupplydecreasingduringlatepregnancy,maternalbodyweight weresignificantly reducedinRG2,RG1groups（p0.01）.Maternalnetbodyweightloss ofeweswere7.24kg（p0.01）andtissuesoftheewesweremobilized14.24%inRG2 group（p0.01）;Maternalnetbodyweightloss of eweswere14.24kg（p0.01）and tissues of the ewes were mobilized 22.91 % in RG1 group（p0.01）. The GLUconcentrationofewesinbloodweresignificantlydecreasedinRG1groupwhen comparedtoRG2,CGgroups（p0.01）, onthecontrary,theBHBAconcentrationwere significantlyincreasedwhencomparedtoRG2,CGgroups（p0.01）andtheNEFA concentrationweregreatlyhigherthanthoseofRG2（p0.05）,CG（p0.01）groupsat120dofgestation;TheGLU,TAAandALBconcentrationweregreatlylowerinRG2, RG1groups thanthoseofCGgroup（p0.01）andtheTPand GLBconcentrationinRG1 groupweresignificantlydecreasedcomposedwithCGgroup（p0.01）,however,the NEFAconcentrationinRG2,RG1groupswerehigherthanthoseofCGgroup（p0.01）andtheBHBAconcentrationinRG1groupwasincreasedcomposedwithRG2,CG group（p0.01）.ThefetalweightandADGweresignificantlydecreasedinRG2,RG1 groupswhencomposedtoCGgroup