HPLC法同时测定双黄连制剂中绿原酸、连翘苷、黄芩苷.PDFVIP

Drug Evaluation Research 第 35 卷 第 2 期 2012 年 4 月 - 113 - HPLC 法同时测定双黄连制剂中绿原酸、连翘苷、黄芩苷 1 1 1 2 2 方海红 ，赵柳入 ，吴杰连 ，饶 毅 ，魏惠珍 1. 江西科技师范学院药学院，江西 南昌 330013 2. 中药固体制剂制造技术国家工程研究中心，江西 南昌 330006 摘 要：目的 建立同时测定双黄连制剂中绿原酸、连翘苷和黄芩苷的 HPLC 方法。方法 色谱柱为 Agilent ZORBAX XDB C18 柱（250 mm ×4.6 mm ，5 μm ）；流动相为甲醇-0.2%磷酸溶液，线性梯度洗脱，体积流量为 1.0 mL/min，检测波长为 324、 280 nm ，柱温 30 ℃。结果 绿原酸、连翘苷、黄芩苷的线性范围分别为 0.093～1.860、0.022～0.440、0.328～6.560 μg，平 均加样回收率分别为 98.8%、98.4%、99.3% 。结论 该方法简便、快速、准确，可作为双黄连制剂的质量控制方法。 关键词：双黄连制剂；双波长高效液相色谱法；绿原酸；连翘苷；黄芩苷 中图分类号：R927.2 文献标志码：A 文章编号：1674 - 6376 (2012) 02 - 0113 - 04 Simultaneous determination of chlorogenic acid, forsythin, and baicalin in Shuanghuanglian preparations by dual-wavelength HPLC 1 1 1 2 2 FANG Hai-hong , ZHAO Liu-ru , WU Jie-lian , RAO Yi , WEI Hui-zhen 1. College of Pharmaceutical Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China 2. National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Nanchang 330006, China Abstract: Objective To establish an HPLC method for simultaneously determining chlorogenic acid, forsythin, and baicalin in Shuanghuanglian preparations. Methods HPLC assay was carried out using an Agilent ZORBAX XDB C 18 column with column temperature at 30 ℃; The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution with flowing rate of 1.0 mL/min; And the detection wavelengths were 324 and 280 nm. Results The linear ranges of chlorogenic acid, forsythin, and baicalin were 0.093—1.860, 0.022—0.440, and 0.328—6.560 μg respectively, and the average recoveries of three components were 98.8%, 98.4%, and 99.3% respectively. Conclusion The method is simple, quick, and accurate, and may be used for the quality control o