一测多评法测定菊苣中绿原酸秦皮乙素异绿原酸B和异绿原酸A的量.PDF

• 666 • 中草药 Chinese Traditional and Herbal Drugs 第 47 卷 第 4 期 2016 年 2 月 一测多评法测定菊苣中绿原酸、秦皮乙素、异绿原酸 B 和异绿原酸 A 的量 朱春胜，林志健，牛红娟，王雪洁，王红坡，孙小霞，王 雨，周 月，聂安政，肖明良，张 冰* 北京中医药大学中药学院，北京 100029 摘 要：目的 建立菊苣 Cichorium intybus 中绿原酸、秦皮乙素、异绿原酸B 和异绿原酸 A 的一测多评方法。方法 以秦 皮乙素为内参物，分别建立绿原酸、异绿原酸 B 和异绿原酸 A 的相对校正因子，并用该校正因子进行绿原酸、异绿原酸 B 和异绿原酸 A 量的计算（计算法），实现一测多评；同时采用外标法测定菊苣中 4 种成分的量（实测法），并比较计算值与 实测值间的差异性，验证所建立方法的准确性、适用性和重复性。结果 8 批菊苣中绿原酸、异绿原酸 B 和异绿原酸 A 量 的计算值与实测值无显著差异。实验建立的菊苣中绿原酸、秦皮乙素、异绿原酸 B 和异绿原酸 A 的一测多评法准确性、可 行性、重复性好。结论 一测多评法测定菊苣中绿原酸、秦皮乙素、异绿原酸 B 和异绿原酸 A 的质量评价模式得到了验证， 可用于菊苣药材的质量控制。 关键词：一测多评；菊苣；绿原酸；秦皮乙素；异绿原酸 B ；异绿原酸A 中图分类号：R286.6 文献标志码：A 文章编号：0253 - 2670(2016)04 - 0666 - 05 DOI: 10.7501/j.issn.0253-2670.2016.04.023 Simultaneous determination of chlorogenic acid, aesculetin, isochlorogenic acid B, and isochlorogenic acid A in Cichorii Herba by QAMS ZHU Chun-sheng, LIN Zhi-jian, NIU Hong-juan, WANG Xue-jie, WANG Hong-po, SUN Xiao-xia, WANG Yu, ZHOU Yue, NIE An-zheng, XIAO Ming-liang, ZHANG Bing School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100029, China Abstract: Objective To establish the quantitative analysis of multi-components by single marker (QAMS) method to determine the content of chlorogenic acid, aesculetin, isochlorogenic acid B, and isochlorogenic acid A in Cichorii Herba (chicory). Methods Selecting aesculetin as the internal reference substance, the relative correction factors (RCF) of chlorogenic acid, isochlorogenic acid B and isochlorogenic acid A were established respectively, and then the contents of the three constituents were calculated by RCF, to achieve the quality of chicory through QAMS. At the same time, the external standard method was used to determine the content of four constituents in chicory and compare the difference between calculated values and measured values, so as to verify the