小型三维激光荧光系统-信号与信息处理专业论文.docx

SmallThree-dimensionalLaserFluorescenceSystemAbstractDifferentalgaecontaindifferentpigments,resultingindifferencesintheir fluorescencespectrum.TheFluorescencespectracanbeobtainedbyfluorescence detectiondevicesandthenusedforclassificationonthealgae.Conventional fluorescencedetectiontypicallyusePMTarrayasthespectraldetector,although havingahighspectralresolution,butverybulkytoo.Inaddition,becausethe exposuretimecannotbeadjusted,thedetectionofthefluorescenceinthelower concentrationofseaweedisnotideal.Thispaperpresentsthedesignoftheminiature spectrometerbased on linear CCD with continuouslyadjustableexposuretime.TheusesofSNOY’s2048-pixelCCDlinearsensorasthespectraldetectorand optical system based onCzerny-Turnerstructure reducetheinstrument’svolume effectively.Accordingtothisdesign,oneofAltera’sCycloneIIIseries FPGA-EP3C25F324isusedasthesystemcontrolcore.Thetiming-drivenmoduleof CCDandADC,accumulatormoduleandaccumulatorcontrolmoduleare implementedinthelogicunitsofFPGA.BasedontheSOPC,NIOSIIsoftcore processor,serialcommunicationmoduleandSDcardfilestoragemoduleare integrated.Thesoftwareaboutsystemparametersset,spectracollectionandanalysis is developed based onLabVIEW.Atlast,theimitationexperimentaboutthedesignshowsthattheminiaturized spectrometercanachievecontinuouslyadjustingexposuretimeand multi-wavelengths measurement simultaneously.Keywords:fluorescencedetection;miniaturespectrometer;lineararray CCD; FPGA; LabVIEW目录1 绪论...................................................11.1引言........................................................11.2 荧光的产生机制..............................................11.3 用于海洋浮游藻类检测的荧光仪器的国内外发展现状..............31.3 论文的研究内容和意义........................................42基于线阵CCD的微型光谱仪的设计方案52.1 光谱仪器的工作原理..........................................52.2 CCD的基本工作原理62.3 微型光谱仪光学系统设计......................................63 微型光谱仪系统的硬件电路设计...........................83.1 硬件电路设计总体方案........................................83.2 关键芯片选型.....................................