牛粪强制通风静态堆肥中添加石灰氮对大肠杆菌的杀灭效果及相关指标的测定-养殖专业论文.docxVIP

The Effect of Adding Lime Nitrogen to Cattle Manure Forced Aerated Static Composting on Killing Escherichia coli and the Determination of Related IndicatorsAbstractEscherichia coli normally does not cause the animal incidence, but some specific serotypes of E. coli pathogenic to humans and animals．In this experiment,the scene cows fresh fecal samples collected, E. coli were isolated，biochemical identification,animal pathogenicity test and serotype identification with special serotypes of pathogenic E. coli, and then through the collection with different serumE. coli cow manure on-site aerated static compost. Add lime nitrogen in the compost, to study the antibacterial activity of different serotypes of bovine pathogenic E. coli. Throughout the study and the results are as follows:69 suspected E. coli isolated from the header Houjiaying dairy 77 apparently healthy adult lactating Holstein dairy cow feces, 12 is a strong pathogenic E. coli were identified, accounting 17.4% of the separation of bacteria, while 12 of highly pathogenic Escherichia coli anti-O serum identification, belonging to 7 serotypes accounted for 25.0% of the stereotypes strains, 8.3%, 16.7%, 8.3%, 8.3%, 8.3%,25.0%.O78, O6, O9, O98, O1, O8, O149 7 kinds of serotypes of E. coli cow feces collected with on-site aerated static compost, composting set to control group and add 2% lime nitrogen test group, 63d compost. The results showed that: the experimental group supplemented with 2% lime nitrogen compost 26d temperature is above 600C and maintained for 9 days, The compost 21d have not detected E. coli and compost the end of the GI value of 108.10 ± 2.44, but did not add 0% group of lime nitrogen in the compost 9d temperature began to rise, reaching more than 50OC, E. coli is not detected when the compost 28d, the GI maximum value of 90.40 ± 2.19, two sets of pH range between 8 and 9.0.composting is a microbial-mediated biochemical processes, so the composting process, carbon and nitrogen changes coul