体外模拟胃肠消化中山楂多酚及抗氧化活性的变化-河南农业科学.pdfVIP

2018-01-22 体外模拟胃肠消化中山楂多酚及抗氧 化活性的变化 陈希苗，李美英，许秋莉，熊楚欣，孙远明* （华南农业大学食品学院，广东省食品质量与安全重点实验室，广东 广州 510642 ） 1 摘 要：本文研究了山楂多酚、黄酮及抗氧化活性在体外模拟胃肠道环境下的变化规律，并利用高效液相色 谱法测定了胃肠消化对山楂中 5 种主要单体酚类化合物的影响。结果表明：山楂多酚在模拟胃液消化中稳 定性较好，总多酚、黄酮含量及抗氧化能力均无显著性变化（P0.05 ），而在模拟肠液消化中山楂多酚易发 生降解，总多酚、黄酮含量在 0.5 h 内分别降低了 16.26 %和 7.46 % ，抗氧化能力也随之降低了 10.25 %。 高效液相色谱测定结果显示，5 种单体酚类化合物含量在胃液消化中无显著性变化（P0.05 ），在肠液消化 中变化却不相一致，其中金丝桃苷和异槲皮苷含量无显著性变化（P0.05 ），表儿茶素在0.5 h 内完全降解， 绿原酸和原花青素 B2 在 0.5 h 内分别显著下降了40.78 %和 28.99 % 。 关键词：山楂；体外模拟胃肠消化；多酚；抗氧化 Changes in polyphenols contents and antioxidant activity in hawthorn (Crataegus pinnatifida Bunge) during simulated gastrointestinal digestion CHEN Ximiao, LI Meiying, XU Qiuli, XIONG Chuxin, SUN Yuanming* （College of Food Science, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China ） Abstract: This paper was aimed to investigate the changes in the contents of total polyphenols and flavonids, as well as antioxidant activity of hawthorn during simulated gastrointestinal digestion. The effect of simulated gastrointestinal digestion on 5 main phenolic compounds was also determined using high performance liquid chromatography (HPLC). The results showed that polyphenols of hawthorn were stable during gastric digestion and the contents of total polyp