生物技术毕业论文汇.docVIP

生物技术毕业论文 专业： 生物技术 SSR分子标记在核桃遗传分析中的应用 摘 要 目的 利用SSR技术对黑核桃、元丰实生核桃、云新核桃、泰山野核桃和陕西实生核桃进行种质资源的遗传分析。 方法 改良的CTAB法提取基因组DNA,在建立了良好的PCR反应体系基础上,利用筛选出来的8对引物对30份材料进行PCR扩增及聚丙烯酰胺凝胶电泳,聚类分析。 结果 在相似系数0.682时,可将供试的30份材料分为4个类群（SSR groups，简称Sg）SgⅠ类包括12份材料,占供试材料的40.00%。SgⅡ、Ⅲ、Ⅳ类各包括核桃材料的6份,分别占20.00%。SgⅠ类先和SgⅡ类群聚到一起,再和其他三类聚到一起。当相似系数为0.793-0.863之间时,又可将SgⅠ类群分为2个亚群，SgⅠ-1和SgⅠ-2，SgⅠ-1中包括所有的元丰核桃实生后代；SgⅠ-2中包括所有的陕西核桃品种。SgⅡ包括所有的云新核桃品种,由于采集的样本来源较近,所有的材料可能是同一种品种。SgⅢ、Ⅳ分别包括所有的泰山野核桃和黑核桃,而且遗传复杂性高。 结论 元丰实生后代、陕西核桃与云新核桃遗传关系最近。云新核桃遗传相似系数为1,可能取材来自同一种样本。泰山野核桃和以上三者有较大的差别,遗传关系较远。黑核桃是遗传特性差别更大,最后才相聚。 关键词： SSR ( Simple Sequence Repeat)；核桃；遗传分析 Abstract Purpose: This Experiment use of this technology on the black walnut, seedling Yuanfeng, Yunxin, Taishan wild walnut and Shanxi walnut in Genetic analysis of germplasm resources. Methods:Extracting genomic DNA by Modified CTAB method?, In the establishment of a sound base on PCR reaction system. Using of screening out of eight pairs of primers in 30 materials amplified PCR and acrylamide gel electrophoresis,then Cluster Analysis.? Results?: 30 materials fall into 4 Groups(SSR-PCR, Sg).SgⅠconsists of 12 materials which account for 40.00%.Every species of SgⅡ,Ⅲ,Ⅳ consists of 6 materials that account for 20.00%. SgⅠand SgⅡget together first ,then get together with the rest species. SgⅠfalls into 2 subgroups, that is SgⅠ-1 and SgⅠ-2. SgⅠ-1 consist of all descendants of Yuanfeng walnut. SgⅠ-2 consist of all species of Shanxi walnut. SgⅡconsist of all species of Yunxin walnut. Because of the source of the collection of swatch is close. Maybe all the materials of Yunxin walnut are the same variety. SgⅢ consist of all theTanshan wild walnut and SgⅣ consist of all the black walnut. The two species of all the materials’complexity are high in heredity. Conclusions:The genetic relationships?among Yuanfeng Seedling generations?,Shanxi walnut and Yunxin walnut. Genetic similarity coefficient of Yunxin walnut is 1,the drawn maybe from the same sample.That are differences between