毕业论文--基于纳米金团聚非标记比色检测过氧水.docVIP

目 录 摘要 1 1引言 3 2实验部分 6 2.1 试剂与仪器 6 2.2 纳米金的制备 6 2.3 实验方法 6 3 结果与讨论 7 3.1 基本原理 7 3.2 比色检测 8 3.3 紫外可见吸收光谱 9 3.4 实验条件的优化 10 3.4.1 邻苯二胺浓度的影响 10 3.4.2 HRP酶浓度的影响 10 3.4.3 吸附时间的影响 11 3.4.4 反映温度的影响 11 3.5 传感器的响应性能 12 3.6 回收率 14 4 结论 14 参考文献 15 致谢 17 基于纳米金团聚非标记比色检测过氧水 摘要 未的金纳米粒子的比色法过氧化氢（双氧水）。邻苯二胺过氧化物酶的过氧化氢，10分钟未的金纳米粒子作为“反应”加入到反应溶液中。混合颜色从红色变到蓝色。辣根过氧化物酶偶氮苯。使用此方法，过氧化氢可以在约4个数量级的浓度范围内半定量。如果420 nm处峰强度，可以准确测定1.3×10-6 M的过氧化氢。与传统的电化学相比，这种传感几个重要的优点：（1）能够（2）避免金纳米（3）。特别值得注意的是这种高效便捷的策略也适用于其他的检测，如和胆固醇。 比色法金纳米粒子辣根过氧化物酶邻苯二胺 Homogeneous, unmodified gold nanoparticle-based colorimetric assay of hydrogen peroxide Zhao Min Department of Chemistry and Chemical Engineering, Class 08102 Abstract: An unmodified gold nanoparticle-based colorimetric assay system in homo- geneous format has been developed using hydrogen peroxide (H2O2) as a model analyte. H2O2 is added to o-phenylenediamine/horseradish peroxidase solution, and allowed to react for 10 min. Then, unmodified gold nanoparticles that serve as “reaction indicators” are added to the reaction solution. The resulting mixture color changes dramatically from red to blue. The reason is that azoaniline, a horseradish peroxidase-catalyzed oxidation product, induces the nanoparticle aggregation. Using this approach, H2O2 can be semiquantitatively determined over the concentration range of ~4 orders of magnitude by the naked eye. If the observed peak intensity at 420 nm is used for the construction of the calibration plot, hydrogen peroxide can be accurately determined down to concentration levels of 1.3×10?6 M. Compared with the conventional electrochemical protocol, this sensing system offers several important advantages: (1) ability to be monitored by the naked eye, (2) avoiding the need of surface modification of electrodes or gold nanoparticles and (3) detection in homogeneous solution. It is worthy of note that this efficient and convenient strategy is also suitable for the detection of other species, such as glucose and cholesterol. Keyword