08第八章反应器与传感器.ppt

反应器及其配件 气升式反应器 填充床反应器 流化床反应器 扩张床反应器 EBA的操作方法 流化床反应器 Sensors and probes 尾气分析 * * 机械搅拌罐 2升试验罐 15升试验罐 中试规模发酵罐 工业规模发酵罐 Turbine impeller Paddle impeller 圆盘六直角叶涡轮搅拌器 螺旋浆搅拌器 斜叶浆搅拌器 螺带浆 锚式浆 Chemineer asymmetric deep hollow blade BT-6 impeller Lightnin A340 hydrofoil impeller Prochem Maxflo (MF) hydrofoil impeller Air-lift Bioreactor Types Air-lift internal cross-flow bioreactor(30,150L) pilot plant 工业规模的气升式反应器 SV FB EB PB Stirred vessel, Fluidized bed, Expanded bed, Packed bed fluidized bed bioreactor (E500) for the cultivation of mammalian cells Despite the small volume of Celonic bioreactor (E2500), its productivity is comparable to a 150-litre stirred tank bioreactor. Features of fluidized bed bioreactors: High-density cultivation of mammalian cells (1011 cells/500ml) GMP-compatible Suitable for anchorage-dependent and suspension cells Bench-top bioreactors Easy to operate Sterilisable in an autoclave Product pipeline from small to large scale (E10 to E2500) (A) Mechanically agitated bioreactors: (a) stirred tank reactor equipped with various propellers (spin, helix, bladed, paddle), (b) rotary drum tank　reactor; (B) Air driven bioreactors: (a) bubble column, (b) concentric tube airlift reactor (IL ALR), (c) external loop airlift reactor (EL ALR), (d) propeller loop reactor, (e) jet loop reactor; (C) Non-agitated bioreactors: (a) packed bed, (b) fluidized bed, (c) membrane reactor. Bioreactor types for plant cell, tissue and organ cultures Synthecon Rotary Cell Culture System Bioreactor for Animal Cell miniPERM bioreactors on a bottle turning device Wave Bioreactor Cellmax? artificial capillary cell culture system TECNOMOUSE system Gas-permeable bag Hollow fiber bioreactor circuit Hollow fiber bioreactor The gas is sparged at the bottom of the draft tube. The bubbles are prevented from leaving the draft tube at the top because they cannot rise against the downward flow produced by th