茵陈提取黄酮工艺和不同地区黄酮含量分析.docxVIP

茵陈提取黄酮工艺及不同地区黄酮含量分析[摘要]目的：建立茵陈提取黄酮类物质最佳工艺和含量测定方法，为扩大工业化生产提供理论基础。并通过比较不同地区黄酮含量分析。方法：采用紫外分光光度法，以芦丁为对照品，在511.5nm处测定吸光度。通过L9（34）正交试验确定了从茵陈中提取黄酮的最佳工艺条件，用紫外分光光度法测定吸光度，计算其黄酮类物质含量。结果:本文研究茵陈中提取黄酮的工艺，其最佳提取工艺为乙醇浓度40%，提取时间30分钟，固液比1:30，提取温度为60℃，各因素影响大小的顺序是：提取温度固液比乙醇浓度提取时间。不同区域黄酮类物质含量：陕西河北山西，但各个产地茵陈中黄酮物质含量相差不大。结论：该方法简便、精密度、重现性、稳定性好。可用于茵陈提取黄酮工艺的研究。不同产地茵陈中黄酮含量有差别，但相差不大。[关键词]茵陈；黄酮；提取工艺；紫外分光光度计；提取率Extraction and flavonoid of Herba artemisiae scopariae and analysis of flavonoids in different regions[Summary] Objective: to establish a wormwood extract optimum process and determination of flavonoids, provide rationale for the expansion of industrial production. And through a comparative analysis of flavonoids in different parts. Methods: the use of UV spectrophotometry, with rutin as reference substances, measurement of absorbance at the 511.5nm. L9 (34) orthogonal test to determine the optimal technological conditions of extracting flavonoids from Herba artemisiae scopariae, UV-Spectrophotometric Determination of absorbance, calculate the content of flavonoids. Results: this study on extraction technology of flavonoids in wormwood, with extraction of ethanol concentration 40%, extraction time: 30 minutes, 1:30 solid-liquid ratio, extraction temperature of 60 ° c, the factors affecting the size of the order is: extracting temperatures solid-liquid ratio ethanol extraction time. Contents of flavonoids in different regions: Shaanxi Hebei in Shanxi province, but the contents of flavone from Artemisia capillaris matter not that far away. Conclusion: the method is simple, precision, reproducibility, stability is good. Study on flavonoids extraction for wormwood. Contents of flavone from Artemisia capillaris differentiated from different habitats, but not that far away. [Keywords] wormwood; flavonoids; extraction; ultraviolet spectrophotometer; extraction rate目录1.引言11.1研究背景11.2国内外研究状况11.3研究的目的和意义22.实验材料、仪器设备与实验方法22.1材料22.2仪器设备22.3原料的预处理22.4黄酮含量的测定方法23结果与讨论43.1乙醇浓度对提取效果的影响43.2提取时间对茵陈中黄酮类物