肤灵浸膏治疗湿疹的主要药效学分析word格式论文.docxVIP

中文摘要目的：运用动物实验模型观察肤灵浸膏抗过敏、止痒、抑菌的作用，初步探讨其作用机制。方法：1．用大鼠被动皮肤过敏法建立I型变态反应动物模型，观察大鼠背部蓝色反应斑直径、光密度值及其抑制率和大鼠血中血管活性物质TXB2、6-keto-PGFla含量及其比值；2．用组胺致豚鼠局部瘙痒法，观察出现豚鼠回头添擦伤时所给予的磷酸组胺总量；3．用纸片扩散法和琼脂平板稀释法观察肤灵浸膏的体外抑菌作用。结果：1．肤灵浸膏各剂量组能明显降低模型组大鼠蓝斑直径和光密度值（p0.05）；高中剂量组能明显降低模型大鼠血浆中TXB2、6-keto-PGFla的含量，以降低6-keto-PGFla的含量为主（p0.05）；肤灵浸膏中剂量组能显著调节模型大鼠血浆中TXB2/6-keto-PGFla的比值（p0.01），表明肤灵浸膏对I型变态反应有抑制作用。2．肤灵浸膏各剂量组的致痒阈显著高于模型组致痒阈（p0.05），表明肤灵浸膏具有较显著的局部止痒效果。3．肤灵浸膏对金黄色葡萄球菌、大肠杆菌、表皮葡萄球菌、沙门氏菌、绿脓杆菌的抑菌圈直径分别为13cm、12cm、9cm、8cm、7cm；对金色葡萄球菌最小抑菌浓度(MIC)为250mg/ml，对大肠杆菌MIC为500mg/ml，对表皮葡萄球菌、沙门氏菌、绿脓杆菌菌MIC为1000mg/ml，表明肤灵浸膏有体外抑菌作用。结论：肤灵浸膏能有效对抗I型变态反应，具有止痒和体外抑菌作用。关键词：肤灵浸膏；湿疹；药效学；抗过敏试验；止痒试验；抑菌试验AbstractObjective:Toobservetheanti-allergic，antipruritic，bacteriosticeffectsof Fulingjingaoand exploreits mechanism byusinganimal experimental model.Methods:Firstly，establishtype1allergicSDratmodelbythemethodofratPCAto observethe diameterof theblueplaque，the optical densityand its inhibitory rateontheratbackandthecontentsandratioofthevasoactivesubstancesTXB2 and6-keto-PGFlaintheratblood;Secondly，observetheamountofhistaminephosphatewhentheguineapiglicksitsitchingplacebythemethodofthelocal itchingofguineapigcausedbyhistaminephosphate;Thirdly，observethe bacteriostaticeffectinvitroofFulingjingaobyfilterpapermethodandagarplatedilution method.Results:Ⅰ.AlldosegroupsofFulingjingaocanobviouslydecreasethe diameteroftheblueplaqueandtheopticaldensityofSDratsinthemodelgroup (p0.05)；High and medium doses of Fulingjingao can obviouslyinhibit thecontent of theTXB2 and 6-keto-PGFla in the blood ofSD rats in the model group ，especiallythecontentof6-keto-PGFla(p0.05)；MediumdoseofFulingjingao canadjusttheratiooftheTXB2and6-keto-PGFlaofthevasoactivesubstancesinthe bloodofSDratsinthemodelgroupsignificantly(p0.01)，whichmeansFulingjingaocaninhibitthetype1hypersensitivity.Ⅱ.TheitchingthresholdofallthedosegroupsofFulingjingaoisobviouslylargerthanthatoftheModelgroup(p0.05) ，which means Fuling jingao can stop local itching effectively. Ⅲ.Inhibition zone diam