基于nmr代谢组学方法分析脑脉通治疗脑缺血再灌注模型的分子机理-analysis of molecular mechanism of naomaitong in treating cerebral ischemia-reperfusion model based on nmr metabonomics method.docxVIP

目录中文摘要..............................................................IABSTRACT...........................................................III序言................................................................11 复方脑脉通研究基础..................................................11.1中医研究缺血性脑中风的意义.......................................11.2复方脑脉通研究现状...............................................12代谢组学研究概述...................................................42.1 代谢组学概念...................................................42.2代谢组学测定方法................................................52.3代谢组学数据处理方法.............................................52.3.1化学计量学与模式识别技术.....................................52.3.2 主成分分析..................................................计算协方差矩阵.............................................求出Σ的特征值?i及相应的正交化单位特征向量ai选择主成分................................................计算主成分载荷.............................................计算主成分得分.............................................82.4代谢组学测定方法的应用...........................................83.4.1 药物的临床安全性评价........................................113.4.2 疾病诊断研究...............................................124 总结..............................................................13第一章复方脑脉通治疗脑缺血/再灌注模型大鼠病理学研究..................15前言................................................................151 实验材料...........................................................151.1动物...........................................................151.2药物...........................................................151.3试剂与仪器.....................................................162 实验方法...........................................................162.1动物分组.......................................................162.2大鼠脑缺血/再灌注模型制备.......................................162.3大鼠神经功能缺损体征观察与评分..................................172.4大鼠脑切片制备..................................................173 实验结果...........................................................173.1大鼠神经功能症状评分...................