基于web端可视化rna-seq数据分析平台的开发-development of visual rna - seq data analysis platform based on web terminal.docxVIP

基于web端可视化RNA-seq数据分析平台的开发摘要随着高通量二代测序技术的发展，越来越多的物种和样本完成测序。基于高通量二代测序的转录组学研究也得到了巨大的飞跃，如何高效合理地处理分析这些指数级增长的海量RNA-seq数据则成为了生物研究的瓶颈。RNA-seq 的分析软件和平台近几年已有了很多，但这里面存在着很多问题，另外对于产业化而言，随着个性化测序、云计算、大数据时代的到来，分析软件工具本身可能不是技术的难点.本研究编写了一个基于java语言统一格式编写的用户友好型本地平台，平台整合了RNA-seq中针对转录本各个分析流程中较为主流优质的软件，并把这一平台进一步开发迭代至web端，实现了方便、快捷、高效、可视化的云端RNA-seq分析流程。该web端RNA-seq分析平台进一步使得生物科学家在做转录组学测序分析研究的时候不再被冗杂的软件操作所束缚，帮助他们实现远程一站傻瓜式可视化的 RNA-seq及其他测序数据分析，让他们把更多精力集中到解决生物学问题和挖掘生物学意义上去。关键词：RNA-seq数据分析，web平台，可视化DEVELOPMENT OF VISUAL RNA-Seq DATA ANALYSIS PLATFORMBASEDONWEBABSTRACTWiththedevelopmentofhigh-throughputsecondgenerationsequencing technology,moreandmorespeciesandsamplescompletesequencing,sequencing costfurtherreduces,andbiologyresearchhasenteredtheeraofbigdata. Transcriptomestudiesbasedonhigh-throughputsecondgenerationsequencingalso got ahugeleap: asan important and mature analysis method in transcriptome research, theRNA-seqiswidelyusedinquantitativeanalysisofgeneexpression,alternative splicingvarianceanalysis,lookingforanewtranscriptandsoon.Extractingthe informationoftranscriptomesequencingdataisnolongeraproblemforbiological scientists,buthowtoefficientlyandreasonablyanalyzemassiveamountsofRNA- seq data in exponential growth has becomethe bottleneck of biological research.TherehavebeenalotofRNA-seqanalysissoftwareandplatformsinrecent years,butthereexistmanyproblems,suchasmostplatformsareintegratingvarious third-partysoftware,whichresultsinnonstandardandnotunifiedcallings.Alsomany platformscanonlystart fromacompleteprogramandcannotfromanystep.Andalot ofsoftware’svisualizationeffectispoor.Scientistswhoarenotfamiliarwith bioinformationdontknowwhichkindoftooltochooseforspecificanalysis.Evenif scientistsknowthetoolchoice,complexityofoperationanddebuggingparameters alsoconsumealotoftimecost.Especiallywhendoingcomprehensiveanalysis, softwareandtoomanymodulesmakealotoftroubletoanalysis.Otherwisefor industrialization,withpersonalizedsequencing,cloudcomputing,andtheadventof theeraofbigdata,analysissoftwareit