七氟醚对神经元缺氧损伤的保护及其作用机制.pdf

534 2007 6 29 6 Hebei Medical Journal, Jun 2007, Vol 29, No. 6 ## 王秋筠 姚尚龙 = 观察七氟醚对大鼠海马神经元缺氧损伤的保护作用及其机 用原代培养 的新生大鼠海马神经细胞建立缺氧模型, 用MTT 法测定神经元存活率采用激光扫描共聚焦显微镜动 态监测单 个海马神经元[ Ca2+ ] i 随缺氧或加入KCl 前后的实时变化 七氟醚在 2MAC( 肺泡气最 小有效浓度) , 可降低神经元缺氧损伤时的细胞死亡率( P 0. 05) 0. 5~ 2MAC 七氟醚能抑 缺氧及 50 mmoPl L KCl 引起的[ Ca2+ ] i 升高( P 0. 05) 七氟醚对离体大鼠海马神经元缺氧性损伤具有 保护作用, 其机 可能与七氟醚抑 缺氧引起[ Ca2+ ] i 异常升高有关 = 海马神经元; 缺氧; 钙离子; 七氟醚 Protective effects and mechanisms of sevoflurane on anoxia injuryin cultured rat hippocampal neurons WANG Qiuj un, YAO Shanglong. Department of Anesthesiology, Third Hospital of Hebei edical University, Shjiiazhuang 050000 =Abstract Objective To investi ate the protective effects and its mechanism of sevoflurane on cultured rat hippocampal neurons subjected to anoxia injury. Methods Hippocampal neurons of neonatal rat which had been cultured in vitro for 10 days, were divided into control roups and sevoflurane treatment roup. The neurons were exposed to oxy en- lucose deprivation for 24 h. The cell survival rate in each roup was evaluated by MTT colorimetry .The effect of sevoflurane on neuronal calcium overload evoked by anox ia or 50 mmoPl L KCl or 1 mmoPl L lutamate was observed. The fluo-3, a fluorescent probe, was used for ima in intracellular calcium and laser scannin confocal microscope( LSCM) was used to measure rea-l time chan es of [ Ca2+ ] i in cultured rat hippocampal neurons. Results The hippocampal neurons developed acute neuronal swellin and wide spread neuronal de eneration followin anoxia for 24 h. Sevoflurane at concentration of 2MAC obviously relieved the neuronal injury ( P 0. 05) . Sevoflurane at concentration of 0. 5~ 2MAC si nificantly i