妊娠中期弓形虫感染对胎鼠生长发育影响.docVIP

妊娠中期弓形虫感染对胎鼠生长发育影响 【摘要】目的探讨弓形虫感染妊娠中期BALB/c小鼠对胎鼠生长发育以及胎盘系数的影响。方法在妊娠第8 d采用腹腔注射法建立弓形虫感染的动物模型，于妊娠第12，14，16，18 d分批处死孕鼠，分离胎盘胎鼠，解剖显微镜下观察胎鼠存活情况，称量胎盘和胎鼠重量，测量胎鼠体长和尾长。结果随着妊娠天数的增加，实验组孕鼠吸收胎明显增多，出现死胎和流产等，正常对照组未见异常；正常对照组胎鼠重量、体长和尾长增加明显，胎盘重量呈现先增后减的趋势，胎盘系数呈降低趋势，实验组变化趋势不及正常对照组明显，组内比较差异均有统计学意义(P＜0.05)。相同时间点的比较显示，除12 d的胎盘胎鼠重量14，16 d的胎盘系数和14 d的胎鼠体长外，差异有统计学意义(P＜0.05)。结论弓形虫感染妊娠中期BALB/c小鼠可导致胎盘功能障碍，胎鼠生长发育受限，且随着感染天数的增加，可出现死胎和流产等病理性妊娠的发生。 【关键词】 刚地弓形虫；胎盘系数；体长；尾长Effect on growth and development of fetus infected with Toxoplasma gondii during second trimester pregnancy in rats RAO Huaxiang,HOU Yuying,LIU Zhishen,et al.Institute of Medical Parasitology,Shanxi Medical University(Taiyuan 030001,China) Abstract： Objective To investigate the growth and development of fetus and placental coefficient of BALB/c mice infected with Toxoplasma gondii during the second trimester pregnancy.Methods To establish the animal models by intraperitoneal injection(i.p.) method at the 8th day of pregnancy,then separate the placentas and fetus at the 12th,14th,16th,18th day,respectively.The survival rate of fetus was observed by anatomical microscope.The weight of placentas and fetus were weighted by electronic balance,and the body length and tail length were measured by sliding caliper.Results It was showed that the number of absorption fetus increased apparently along with that of the pregnant days in the experimental group,of which the death of fetus and abortion were more than that of the normal control group.In the normal control group,the weight,body length and tail length of fetus were increased obvious and the placental weight was increased first then decreased,which caused the decrease of placental coefficient.However,the change of fetus and placenta of the experimental group were not obviously compared with the normal control group.At different time points,there was significant difference in each group (P＜0.05).At the same time points,the results of the experimental group were significant different compa