日本血吸虫成虫和虫卵可溶性抗原及其组分抗原的研究.doc

日本血吸虫成虫和虫卵可溶性抗原及其组分抗原的研究 【摘要】 目的： 探讨日本血吸虫雄虫、雌虫、虫卵的可溶性抗原雄虫（AWAm、雌虫AWAf、虫卵SEA）及其组分抗原的特性。方法： 用DE22纤维素层析柱对日本血吸虫AWAm，AWAf，SEA抗原进行纯化，得到相应组分抗原。分别以SDSPAGE和Western blot对组分抗原进行全面分析，并与相应未经纯化的可溶性抗原作比较。结果： 经DE22纤维素层析处理的AWAm，AWAf，SEA,均能获得含多种蛋白的组分抗原。SDSPAGE结果表明, AWAm 见10条主带和9条次带, 其中Mr 37 000,28 000,25 000为特异性条带, 出现8条免疫反应阳性带；而AWAm组分抗原为9条蛋白带和6条免疫反应阳性带。AWAf 见 15条蛋白带,其中Mr 26 500为特异性条带, 组分抗原为8条蛋白带。AWAf粗抗原及其组分抗原均见9条反应带。SEA见8条主带和10条次带,13条为免疫反应阳性条带；SEA组分抗原见11条带,其中9条为免疫反应阳性带。结论： AWAm，AWAf，SEA经DE22纤维素层析纯化获得组分抗原,方法简单、可靠。该组分抗原含有粗抗原的主要免疫反应成分,去除了多种与日本血吸虫病免疫反应无关的蛋白。 【关键词】 日本血吸虫　 可溶性成虫抗原　可溶性虫卵抗原　 组分抗原 Analysis on soluble antigen and its fraction antigen of　Schistosoma japonicum adult and egg JIANG Xugan, FU Xingli, CHEN Shengxia, XU Huijuan,Shuai Lianyun, CAO Jianping, QIU Jinbo (School of Medical Technology, Jiangsu University, Zhenjiang Jiangsu 212013； Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China) ［Abstract］ Objective: To explore the characteristics of soluble male adult worm antigens(AWAm), female adult worm antigens(AWAf), egg antigens(SEA)and their fraction antigens of Schistosomia japonicum. Methods： The fraction antigens were isolated from AWAm, AWAf and SEA on DE22 cellulose column, analyzed by means of SDSPAGE and western blot(WB)．Results： The three types of fraction antigens could be obtained from AWAm, AWAf and SEA on DE22 cellulose column．AWAm and its fraction antigens were separated into 19 and 9 distinct bands in SDSPAGE gel and revealed 8 and 6 immune bands on western blot respectively．AWAf and its fraction antigens were separated into 15 and 8 distinct bands in SDSPAGE gel and displayed 9 immune bands on WB． Most of AWAm and AWAf protein bands were similar, but Mr 37 000 and Mr 28 000 and Mr 25 000 bands were only appeared in AWA and Mr 26 500 band was seen in AWAf． SEA and its fraction antigens were separated into 18 and 11 distinct bands respectively in SDSPAGE gel and showed 13 an