花刺柳珊瑚echinogorgia flora化学成分及生物活性分析-chemical constituents and biological activity analysis of echinogorgia flora from sarcandra glabra.docx

物进行了抗病毒活性测试。结果表明，分离得到的愈创木烷型倍半萜类化合物对肿瘤细胞基本没有抑制活性；化合物（1，2，5，8，9，13）显示弱的抗流感病毒活性！关键词：花刺柳珊瑚；化学成分；生物活性StudiesonChemicalConstituentsandBioactivitiesoftheGorgonianEchinogorgiafloraAbstractThegenusEchinogorgiaisasessileanimaloftheFamilyParamuriceidae,OrderGorgonacea,SubclassOctocorallia,ClassActinozoa,PhylumCnidaria.Thisgenuscontainsabout30species,widelydistributedinthesouthwestofPacificOceanandtheSouthChinaSea.Studiesonchemicalconstituentsofthisgenusbeganinthelate1980sandthesecondarymetabolitesfoundmainlypolyhydroxysterolsaswellasfewothernovelstructuretypesofsesquiterpenesandalkaloids,whichshowedrichchemicaldiversityandpotentialresearchvalueofmarinenaturalproducts.Inodertosearchfornaturalproductswithuniquestructuralfeaturesandunprecedentedbiologicalactivities,wehaveperformedacomprehensiveinvestigationonthechemicalconstituentsandbiologicalactivitiesoftheEchinogorgiafloracollectedfromweizhouislandsintheSouthChinaSea,inOctober,2010.Bybiologicalactivity-orientedisolation,theMeOHextractoftheEchinogorgiaflorawaschromatographedonTLC,silicagel,andLH-20Sephadex,andsemi-preparativeHPLC,respectively.Thenforty-onecompoundswereisolatedandpurifiedfromtheEchinogorgiaflora,amongwichthirty-eightcompoundswereidentifiedbymeansofspectralmethods(UV,IR,MS,NMR,CD,etal.)andtheirphysicochemicalproperties.Amongthemtherearethirteenguaiane-typesesquiterpenes(2~6,7~14);fourteensterols(5~28);onepolyketide(29);oneceramide(30);twoglycerolethers(31~32);onebenzenederivative(33);sixalkaloids(1、34~38).Whatisremarkableisthatanewcarbonskeletonguaipyridinesesquiterpenealkaloidandfivenewguaiane-typesesquiterpenes(2~6)wasobtained.Theknowncompounds(7~33)werefirstisolatedfromtheEchinogorgiafloraandcompounds(8~10,12~25,27~28)werefirstfoundfromthegenusEchinogorgia.Biologicalactivities:Fourteenguaiane-typesesquiterpenecompoundswereevaluatedfortheircytotoxicitiesagainstK562(chronicmyelogenousleukemiacelllineoriginal),A-549(lungcancercellline),HL-60(humanleukemiacells),HeLa(humancervicalcarcinoma),HCT-116(humancoloncance