陈士林中药dna条形码鉴定方法体系的建立与推广.pptxVIP

中药鉴定领域寻求变革与突破;中药鉴定技术瓶颈 ;; Plant Working Group recommended rbcL + matK as the plant barcode, successfully discriminate at the species level --72%. Far from perfect due to the low identification rate PNAS 2009 Science 2009;;课题组首次提出并验证ITS2条形码，引起国际广泛关注; ;Comparative analysis of a large dataset indicates that ITS should be incorporated into the core barcode for seed plantsChina Plant BOL Group, Li Dezhu et al., PNAS, 2011 ;首次验证 ITS2为药用植物鉴定通用条形码;冬虫夏草;Identification of Lycium barbarum and its closely related species using ITS2 barcode;;13;14;常用中药材进行了DNA条形码研究;创建ITS2为主体条形码的中草药鉴定体系;《中国药典》中药材DNA条形码分子鉴定指导原则;建立中药材DNA条形码鉴定系统网站; DNA barcode system shows strong abilities in identifying herb cryptic species BLAST PROCESS;;华润三九中药材DNA条形码鉴定专用软件;2018/5/21; 七叶皂苷注射剂娑罗子药材的基原植物为七叶树Aesculus chinensis、浙江七叶树Aesculus chinensis var. chekiangensis和天师栗Aesculus wilsonii;;;Use of the potential DNA barcode ITS2 to identify herbal materials 全草类DNA条形码鉴定;案例1：为侦破谋杀案提供了关键物证;柴胡 Chaihu;;在google scholar中搜索DNA barcode 共2万条 前3篇 有1篇是中国发表 前20篇有4篇是陈课题组;中药材DNA条形码分子鉴定体系受到国内外广泛关注;A small DNA barcode, a giant leap for taxonomy and species identification条形码一小段 物种鉴定一大步;突破传统分子标记内在鉴定能力瓶颈，绕开核基因组的高复杂度，开发种群水平的、物种特异鉴定标记;High-throughput sequencing of complete chloroplast genomes of Magnolia and their applications ;Plant DNA Barcoding: from gene to genome;Nature Comms: Genome Sequence of the Model Medicinal Mushroom G. lucidum《自然》子刊：基因组解析提出灵芝为首个药用模式真菌;USA Today: Chinese “mushroom of immortality” genome mapped 今日美国评述报道;;A Plea for Pure Science (SCIENCE 1893/8/15);Acknowledgements