il1β诱导肝细胞核受体rxrα核胞浆转移与泛素连接酶gp78表达的相关性分析-correlation analysis between il 1β - induced nuclear cytoplasmic transfer of hepatocyte nuclear receptor rxr α and expression of ubiquitin ligase gp78.docx

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il1β诱导肝细胞核受体rxrα核胞浆转移与泛素连接酶gp78表达的相关性分析-correlation analysis between il 1β - induced nuclear cytoplasmic transfer of hepatocyte nuclear receptor rxr α and expression of ubiquitin ligase gp78.docx

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il1β诱导肝细胞核受体rxrα核胞浆转移与泛素连接酶gp78表达的相关性分析-correlation analysis between il 1β - induced nuclear cytoplasmic transfer of hepatocyte nuclear receptor rxr α and expression of ubiquitin ligase gp78

mRNAMRP2MRP3MTTNTCPmessengerRNAMultidrugresistance-associatedprotein2Multidrugresistance-associatedprotein3MethylthiazolyltetrazoliumNa+/taurocholatecotransporter信使RNA多耐药相关蛋白2多耐药相关蛋白3甲基噻唑基四唑Na+牛磺胆酸共转运蛋白ODPBSPCRPMSFPVDFRpmRNART-PCRRARαRXRαSDSSecTBSTBSTOpticaldensityPhosphatesalinebufferPolymerasechainreactionPhenylmethanesulfonylfluoridePolyvinylidenedifluoriderevolutionsperminuteRibonucleicacidRealtimePCRretinoidacidreceptoralpharetinoidXreceptoralphaSodiumdodecylsulfatesecondTris-bufferedsalineTris-HClTrisbufferedsalinewithTween20光密度磷酸盐缓冲液聚合酶链反应苯甲基磺酰氟聚偏二氟乙烯每分钟转数核糖核酸实时PCR维甲酸受体维甲酸X受体十二烷基硫酸钠秒缓冲盐溶液含Tween20的TBS缓冲液TEB4/MARCHⅣmembrane-associatedRING-CHproteinVITEMEDTrisuMWBTetramethylethylenediamineTris(hydroxymethyl)aminomethaneumol/literWesternBlot四甲基乙二胺三(羟甲基)氨基甲烷微摩尔浓度蛋白免疫印迹杂交ExperimentalstudyontheIL-1βleadtosubcellularre-localizationofhepatocytenuclearreceptorRXRαandexpressionofGP78AbstractBackgroundobjective:Numerousresearchessupportthepointthatabnormalexpressionofmembranetransportproteininlivercellsplaysavitalroleintheonsetofcholestasis.RetinoidXreceptorα(RXRα),amemberofthenuclearreceptorproteinsuperfamily,hascentralregulatoryfunctionintranscriptionofmembranetransportproteinthroughdimerizingwithothernuclearreceptors.RecentstudyprovesthatunderthestimulationofinflammatoryfactorRXRαcouldregulateseveralbileacidtransportproteinexpression,NTCP,BSEP,MRP2andMRP3forinstance,whichprovesitscrucialregulatoryabilityinformationofcholestasis.UnderthecircumstanceofLPSand9cisRA,RXRαintendtotransferintocytoplasmtoexertitsvariousbiologicalfunction.ThescarcityofintracellularRXRαmayresultfromERassociatedproteindegradation(ERAD).ERADparticipatesindegradingmisfoldedproteinandtransformingintracellularproteinwithhighefficiencyandselectivity,besidesthatitinvolvesinPost-translationalmodificationsofcertainsignificantproteins.ubiquitinligase(E3),whichactsasakeyagentinproteosomedegradationpathway,maybeofgreatsignificanceindegradingRXRα.Consequently,wechosethemodelofhumanlivercancercelllineHepG2tr