口虾蛄消化酶及免疫因子特征的初步分析-preliminary analysis of digestive enzyme and immune factor characteristics of mantis shrimp.docxVIP
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口虾蛄消化酶及免疫因子特征的初步分析-preliminary analysis of digestive enzyme and immune factor characteristics of mantis shrimp
盐度的增加表现为先减小后增大,溶菌酶酶活力在盐度27时最小,其余酶活力在盐度30达到最小值。在5-30℃范围内,除了碱性磷酸酶、过氧化氢酶、超氧化物歧化酶活力一直增大外,其余的免疫活性均随着温度的升高表现为先增大后减小,血蓝蛋白浓度、血细胞总数在15℃时达到最大值,酸性磷酸酶、溶菌酶活力分别在25℃、20℃最大,其余酶活力在30℃表现最大。6、饥饿胁迫对口虾蛄成虾免疫活性的影响测定不同饥饿时间对口虾蛄免疫因子的影响,试验结果表明,随着饥饿时间的增加,口虾蛄血蓝蛋白浓度、血细胞总数、磷酸酶活力一直减小,至25d达到最低值;过氧化氢酶、溶菌酶、过氧化物歧化酶活力则表现为先增大后减小的趋势,过氧化氢酶活力在饥饿5d时最大,其余酶活力在饥饿10d时达到最大值。关键词:口虾蛄;消化酶;免疫因子;不同条件AbstractThisdissertationinvestigatedeffectsoflarvalstages,temperature,salinity,starvationanddietsondigestiveenzymeactivities.Itwasstudiedthateffectsoftemperature,salinityandstarvationonimmunefactorsoftotalhemocytecount,hemocyaninconcentrationandlysozymesystemactivitiesinOratosquillaoratoria.Theresultsshowed:1、EffectsofgrowthstagetoO.oratoriaondigestiveenzymeactivityActivitiesofdigestiveenzymesfromlarvalOratosquillaoratoriaⅢ-Ⅺandpo-stlarvaewereanalyzedduringitsontogeneticdevelopment.Theresultsshowedthattherewere3differentpattemsintheactivitiesof5digestiveenzymes.AmylaseactivitywasincreasingfirstlyandreachingapeakatⅤstage;itdecreasedagaininthesubsequentlarvae.Theactivitiesofpepsin,trypsaseandlipaseincreased,whilecellulaseactivitydecreased.Theactivityoflipasewasfairlylow.A/Tratiowasdecreasedgradually.2、EffectsoftemperatureandsalinitytoO.oratoriaondigestiveenzymeactivityItwasshowedthatprotease,amylaseandlipaseactivitywasincreasingfirstlyandthendecreasingwhensalinityrosefrom18to39;cellulaseactivityfluctuatedafterreachingapeak.Cellulase,pepsin,lipaseactivitywashighestatsalinityof27,amylase,trypsinactivitywashighestatsalinityof30.3、EffectsofstarvationtoO.oratoriaondigestiveenzymeactivityItwasshowedthatdigestiveenzymeactivitiesincreasedfirstlyandthendecreasedwithstarvationtimeincreasing.Pepsinactivityreacheditsmaximumonthethirdday;whileotherdigestiveenzymesreacheditsmaximumonthefirstday.Theyallreacheditsminimumonthe14thday.4、EffectsofdietstoO.oratoriaondigestiveenzymeactivityTheresultsshowedthatactivitiesofamylaseandcellulosewerehighest,activitiesofproteaseandlipasewerelowest,andmetamosphsisratewaslowestwhenfeedingchlorella.Diges
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