雷公藤杀虫生物碱的分离、鉴定及应用分析-isolation, identification and application analysis of tripterygium wilfordii insecticidal alkaloids.docx

杭州师范大学雷公藤杀虫生物碱的分离、鉴定及应用研究 杭州师范大学 雷公藤杀虫生物碱的分离、鉴定及应用研究 PAGE PAGE IV Abstract The dissertation was focused on the insecticidal plant, Tripterygium wilfordii Hook. f. First, the insecticidal active compounds of the plant were extracted, separated and identified. Then, some studies, such as bioactivities assay, extraction technical process, analytical methods, production preparation, toxicities accession of these compounds and collection standard of raw materials, were conducted. The main results were as following: The main active constituents of T. wilfordii were sesquiterpenoid alkaloids. Three alkaloids, wilfordine, wilforgine and wilforine, were separated from the extracts. These alkaloids had insecticidal active against some insect, such as Plutella xylostella. The results showed that the three alkaloids were synergistic with each other for that the active of each alkaloid was lower than the total alkaloids, and also the mixture of them. The alkaloids had obviously insecticidal activities to the larvae of Chilo suppressalis, Plutella xylostella, Sesamia inferens and Pieris rapae. The medium values of antifeedance concentration (AFC50) of the total alkaloids against these insects were 188.3, 42.44, 17.42, 22.78 and 46.1, 46.6, 11.44, 41.77 mg/L in choice and non-choice test, respectively. LC50 values of poisoning to these insects were 313.91(48h), 104.99(72h), 15.94(24h) and 148.59 (48h) mg/L, respectively. The medium values of growth inhibiting concentration (EC50) of the total alkaloids against the Chilo suppressalis and Plutella xylostella were 39.38 and 123.3 mg/L. LC50 value of poisoning to Prodenia litura was 144.69 mg/L (48h). The results indicated that the alkaloids had broad spectrum activities against the insects of Lepidoprera, while the rapidly available and the activities against the elderly larvae were not very well. The activity of GSTs in 1st~2nd Plutella xylostella instar larvae increased significantly under treatment with low concentration (LC10)