不平衡的能量摄取与代谢※脂肪细胞病态的增生※高血压第2型糖尿病.PPT

結果 第二十四組 B9732020 張育瑄 (甲) B9732040 吳俊諭 (甲) B9732045 洪姿均 (甲) 指導老師：陳莉臻老師 * 前言 肥胖形成原因 大豆異黃酮 3T3-L1脂肪細胞 大豆異黃酮抑制油滴形成途徑 結論 * 前言 * ※ 不平衡的能量攝取與代謝. ※ 脂肪細胞病態的增生 ※高血壓、第2型糖尿病、 心血管疾病 * (Hwang et al. 2005) * (謝等,2004) ?大豆異黃酮是異黃酮的 一種，在大豆中含量豐富 ?抗氧化活性 ?抗腫瘤 ?預防骨質疏鬆症 * 大豆異黃酮已發現對肥胖、糖尿病、心血管疾病有治療的效果 * (Hwang et al. 2005) differentiation medium * * Effects of genistein on adipocyte differentiation in 3T3-L1 cells. Confluent 3T3-L1 cells were exposed to differentiation cocktail for 2 days, and cells were changed into normal DMEM in the presence or absence of genistein for the indicated concentrations. * (Hwang et al. 2005) Effects of genistein on adipocyte differentiation in 3T3-L1 cells. Confluent 3T3-L1 cells were exposed to differentiation cocktail for 2 days, and cells exposed to genistein for the indicated time period. * (Hwang et al. 2005) AMPK途徑為肥胖和第二型糖尿病相關研究的標靶 AMPK會調節脂肪細胞的脂質新生 * (Luo et al.,2005) * /content/2/4/26/figure/F1 ACC :乙烯輔酶 A羧化酶 malonyl coA :丙二烯輔酶 Afatty acid synthase:脂肪酸合成酶 acetyl-coA carboxylase * /classes/bioc462/462bh2008/462bhonorsprojects/462bhonors2006/warzeckas/metabolic%20regulation.htm The effects of AMPK activation by genistein. 3T3-L1 cells were fully differentiated by differentiation cocktail, cells were exposed to genistein (100 μM) for the indicated time periods. * (Hwang et al., 2005) 結論 * 大豆異黃酮可以藉由活化AMPK以及降低脂肪酸合成酶的表現來抑制3T3-L1脂肪細胞脂質新生 * /classes/bioc462/462bh2008/462bhonorsprojects/462bhonors2006/warzeckas/metabolic%20regulation.htm 參考文獻 * 謝明傑、高爽、鄒翠霞、劉長江、金鳳燮，2004，大豆異黃酮生理功能的研究進展。食品與發酵，30(5):94-98。 Hwang, J. T., Park, I. J., Shin, J. I., Lee, Y. K., Lee, S. K., Baik, H. W., Ha, J., Park, O. J. 2005.Genistein, EGCG, and capsaicin inhibit adipocyte differentiation process via activating AMP-activated protein kinase. Biochem Biophys Res Commun, 338(2):694-699. Rehfeldt, C., Kalbe, C., Nurnberg, G., Mau, M. 2009. Dose-depende