丹红注射液预处理对大鼠缺血再灌注心肌细胞损伤影响.docVIP

丹红注射液预处理对大鼠缺血再灌注心肌细胞损伤影响 　　[摘要] 目的 研究丹红注射液预处理对大鼠心肌细胞缺血再灌注损伤的作用。 方法 体外培养大鼠心肌细胞，将丹红注射液以2％、4％、8％、16％四种浓度分别加入培养基中，预处理24 h后，置于缺血再灌注（I/R）环境中培养，采用流式细胞仪技术检测各组细胞的凋亡率，免疫组织化学法检测凋亡基因Bax及Bcl-2蛋白表达变化。 结果 与正常对照组比较，缺血再灌组细胞凋亡率及Bas表达明显升高，Bcl-2表达则显著降低；而用丹红注射液预处理后呈浓度依赖性地下调凋亡率及Bas表达，上调Bcl-2表达。 结论 丹红注射液对缺血再灌心肌细胞有保护作用，且与浓度有一定的依赖关系，其作用可能是通过上调Bcl-2蛋白表达，抑制Bax蛋白表达实现的。 　　[关键词] 丹红注射液；缺血再灌；心肌细胞；细胞凋亡 　　[中图分类号] R331.3 [文献标识码] A [文章编号] 1673-7210（2012）02（a）-0024-02 　　 　　The effect of Danhong Injection pretreatment on cardiomyocytes cell of ischemic-reperfusion rats 　　ZHENG Yaping HAN Kun 　　Department of Physiology, Luohe Medical College, He′nan Province, Luohe 462002, China 　　[Abstract] Objective To investigate the effect of Danhong Injection pretreatment on cardiomyocytes cell of ischemic-reperfusion rats. Methods The rats cardiomyocytes were cultured and pretreated with Danhong Injection in four different concentrations: 2％, 4％, 8％, 16％ before create cardiomyocytes I/R model after 24 hours for pretreatment. The apoptotic rate of cardiomyocytes was determined by flow cytometry, the protein expressions of Bcl-2 and Bax were observed by imnmnohistochemistry. Results Compared with control group, the apoptotic rates were significantly increased in ischemia-reperfusion (I/R) group, and the protein expressions of Bax expressions was significantly up-regulated and Bcl-2 expressions was down-regulated. Pretreatment of cardiomyocytes with Danhong Injection increased the expression of Bcl-2, decreased the expression of Bax and the apoptotic rate in a dose-dependent fashion. Conclusion Danhong Injection alleviates I/R injury in a concentration-dependent manner. These effects may be partially that Danhong Injection can inhibit Bas gene protein expression and induce Bcl-2 gene protein expression. 　　[Key words] Danhong Injection; Ischemia-reperfusion; Cardiomyocytes; Apoptosis 　　 　　心肌缺血再灌注损伤（isehemia-reperfusioninjury，I/R）是心脏缺血性疾病及心外术后心肌损害的主要原因，其导致的心肌细胞凋亡被认为是再灌注损伤的重要环节。丹红注射液是由丹参与红花萃取制备而成，具有保护心血管的作用，已