ns398对人胃癌细胞系sgc7901增殖 凋亡和cox2表达的影响-effects of ns 398 on proliferation, apoptosis and co x2 expression of human gastric cancer cell line sgc 7901.docxVIP

目录缩略词 表·····································································································(2)中文摘 要·····································································································(3)英文摘 要·····································································································(4)引 言·····································································································(5)材料方法·····································································································(7)3结果·····································································································(15)讨 论·····································································································(20)结 论·····································································································(27)参考文 献·····································································································(28)致 谢·····································································································(33)综 述····································································································4·(34)缩 略 词 表(Abbrevations)FGFfibroblast growth factor成纤维细胞生长因子VEGFvasocular endothelial growth factor血管内皮生长因子DMSOPBSdimethyl sulfoxidephosphate bufferedsaline二甲基亚砜磷酸缓冲盐COXcyclooxygenase环氧合酶COX-1COX-2cyclooxygenase-1cyclooxygenase-2环氧合酶-1环氧合酶-2PGE2HLAprostaglandin E2human leucocyteantigen前列腺素 E2人白细胞抗原MTTmethyl thiazolyl tetrazolium四甲基偶氮唑蓝ELISAICCimmunosorbent assayimmunocytochemical method酶联免疫测定法免疫细胞化学法DABdiaminobenzidine二氨基联苯胺FBSODfetal bovine serumoptical concentration胎牛血清吸光度RPMSPSSrevolution per minutestatistical package for scoial science每分钟转速社会科学统计学软件5NS-398 对人胃癌细胞系 SGC-7901增殖、凋亡和 COX-2 表达的影响中文摘要目的 探讨环氧合酶-2（COX-2）抑制剂 NS-398 对人胃癌细胞系（SGC-7901） 增殖、凋亡及 COX-2 表达的影响，并进一步探讨其作用的可能机制。方法 采用四甲基偶氮唑蓝法(MTT)检 NS-398 对 SGC-7901 细胞的杀伤抑制作 用；免疫细胞化学法检测 SGC-7901 细胞内 COX-2 的蛋白表达情况；酶联免疫 测定法(ELISA)检测 NS-398 作用 SGC-7