(生物工艺学双语课件）Chapter 06Modes of Process Operation教程文件.pptVIP

Chapter 6;i) the batch mode, where Fi=Fo=0 ii) the fed-batch mode, where Fo=0 and Fi=F0 iii) the continuous mode, where Fi=Fo=F0;6.1. Batch fermentation ;;Schematic representation of growth phases in a batch process;;6.2. Continuous fermentation;6.2.1 The chemostat;6.2.2 Presentation of the chemostat properties;The live cell balance equation Change= Input –Outlet +Production - consumption;;The effect of the saturation constant(Ks) of the limiting substrate on the outlet concentration of this compound in a chemostat;The concentration of viable cells (Xv) Change= Input –Outlet +Production - consumption;The concentration of dead cells (Xd) Change= Input –Outlet +Production - consumption;Productivity of cells (rX);Concentration of product (P) Change= Input –Outlet +Production -consumption;The dissolved oxygen tension (DOT) Change= Input - Outlet +Production - consumption;;1. Dilution rate D 2. Inlet limiting substrate concentration Si 3. Recycling ,separation factor;;Advantages Disadvantages Higher productivity Limited to growth related products The physiology can be controlled Increased risk of infecting Steady state conditions Sensitive to genetic instability the time of the batch cultivation (tbatch) ;;Determination of maximum growth rate ,μmax;Yield coefficient of biomass inclusive the maintenance (Yx/s);;6.3. Fed-batch fermentation;Change= Input - Dilution +Production - consumption;;;What to fed-batch ? medium ? carbon source ? How to fed-batch ? constant fed-batch ? exponential fed-batch ? others ?;6.3.2 Control of the fed-batch process;;6.3.3 High cell density cultures;;;;6.3.4 Control of exponential growth in fed-batch cultures;;6.3.5 DOT controlled feeding;;6.3.6 Advantages and limitation ;Constant DOT Constant glucose co