国际森林土壤研讨会课件Fan Dongmei教程文件.pptVIP

Dongmei FAN, Xiaochang WANG* Institute of Tea Science, Zhejiang University Contact Us fandongmei89@126.com Effects of short-term fertilization on the soil polyphenol variation and carbon accumulation in tea (Camellia sinensis.L) garden soils Properties Strong antioxidant capacity Provide a large number of binding sites Three ways into soils Litter decomposition; root exudation and leaching Backgrounds catechins Soil C cycle 1) Stimulating litter decomposition rate and involving in the formation of humic substances 2) Changing microbial community directly or indirectly For soil N cycle, polyphenols have a complicated effects on it Relationship between polyphenols and soil nutrient cycles Backgrounds Tea (Camellia sinensis.L) 18-34% of the dry weight Both tea plants and tea garden soils have a large amount of polyphenols Backgrounds Backgrounds Total soil C stock of tea gardens in China from 1950 through 2010 Historical documents Experimental data Experimental design Fertilizer TC(%) TN (%) Available phosphorous (mg·kg-1) Available potassium (mg·kg-1) Rapeseed cake 42.6 4.5 154.4 1379.10 Chicken manure 15.1 2.0 188.7 985.88 Urea 20.0 46.0 0 0 Nutrient compositions ? Low level （kg·mu-1） Medium level （kg·mu-1） High level （kg·mu-1） Urea 25(N1) 50(N2) 100(N3) Rapeseed cake 255.5(B1) 511(B2) 1022(B3) Chicken manure 575(F1) 1150(F2) 2300(F3) CK 0 0 0 Fertilization levels Totalpolyphenol(Tp) Bound polyphenol(Bp) Modified Folin–Ciocalteu method (Heimler et al., 2006) Polyphenol (Xiaochang W, et al., 2006) FDA hydrolase activity Total carbon(TC) Total nitrogen (TN) Elemental Analyzer. multi N/C 3100Analytikjena, Germany C N Polyphenol oxidase; peroxidase (Montgomery, M.W. et al, 1975) Enzyme activities Chemical Analysis Represents for microbial activity Results Bp variation was different with Tp 2. Significant differences of final polyphenol concentration was found among plots whereas no difference was found in initial v