生物化学与分子生物学实习指导（Practice instruction of Biochemistry and molecular biology）.docVIP

生物化学与分子生物学实习指导（Practice instruction of Biochemistry and molecular biology） Guidance for Biochemistry and molecular biology practice (Chinese version) Practice instruction of Biochemistry and molecular biology Experiment 1. Determination of protein content (colorimetric analysis and spectrophotometry) In experiment two, DNA was extracted from liver of mice Experiment three DNA enzymatic hydrolysis and DNA fragment agarose gel electrophoresis Experiment four modern PCR Technology Isolation and identification of serum anti trypsin (alpha 1 AT) in five experimental subjects Content one salting out method, gel filtration method Content two cellulose ion exchange chromatography, determination of protein content, determination of enzyme activity Content three SDS- polyacrylamide gel electrophoresis Content four summary In experiment six, the specificity, agonist and inhibitor of enzyme were observed Effect of temperature and pH on enzymatic reaction speed in experiment seven Effect of exercise eight on the content of lactic acid in urine Experiment nine liver glycogen test Experiment ten extraction and thin-layer chromatography of liver lipids Experiment 1. Determination of protein content (colorimetric analysis and spectrophotometry) [objective requirements] 1. mastering the basic principle of colorimetric analysis and spectrophotometry, making and using the standard curve, and calculating the result of standard tube method 2. familiar with common methods, principles and applications of protein content determination 3. to understand the principle and application of the commonly used visible and ultraviolet spectrophotometer [teaching content] Determination of protein content by 1. phenol reagent method Lambert-Beer law, colorimetric method and basic principle of spectrophotometry, 722 spectrophotometer, method and application of determination of protein content, protein content determination, the operation principle of phenol reagent method, standard curve preparation and