n乙酰半胱氨酸对pm2.5慢性暴露大鼠肺脏 炎症的干预作用-effect of n - acetylcysteine on lung inflammation in pm 2.5 chronically exposed rats.docx

目录中文摘要············································································································1英文摘要············································································································4研究论文N-乙酰半胱氨酸对PM2.5慢性暴露大鼠肺脏炎症的干预作用前言············································································································7材料与方法································································································9结果··········································································································14附图··········································································································18附表··········································································································24讨论··········································································································28结论··········································································································34参考文献··································································································35附录··········································································································45综述粗、细、超细颗粒物的研究进展····························································46致谢··················································································································56个人简历··········································································································57N-乙酰半胱氨酸对PM2.5慢性暴露大鼠肺脏炎症的干预作用摘要目的：建立大气细颗粒物长期慢性暴露的大鼠模型，观察PM2.5长期慢性暴露大鼠肺脏炎症及氧化应激改变，以及N-乙酰半胱氨酸对大鼠PM2.5慢性暴露导致的肺脏炎症及氧化应激的干预作用。方法：24只SD雄性大鼠（220±20g）随机分成三组，每组8只：PM2.5暴露组（PM组），PM2.5+N-乙酰半胱氨酸（PM+NAC）组，空气净化对照组（FA组）。PM组及PM+NAC组暴露于河北省石家庄市的一间10平方米的房间内,房间窗户开放,保持室内通风，使室内PM2.5浓度与室外相近；分别予以生理盐水1ml/Kg·d及N-乙酰半胱氨酸混悬液150mg/Kg·d灌胃。FA组：将大鼠置于10平方米房间内，门窗保持密闭，室内空气过滤器持续开放，大鼠吸入清洁空气，予以生理盐水1ml/Kg·d灌胃。各组大鼠分别暴露180天，处死。用瑞氏-吉姆萨染色在光学显微镜下计数支气管肺泡灌洗液中白细胞总数、中性粒细胞计数及中性粒细胞百分数；大鼠肺组织石蜡包埋、切片，行H