p16 rassf1a基因甲基化与肺癌及被动吸烟关系的研究-relationship between methylation of p16 rassf 1a gene and lung cancer and passive smoking.docxVIP

目录中文摘要······························································································（1）英文摘要······························································································（2）论文题目······························································································（3）前言···································································································（3）1、材料································································································（5）1.1、实验动物························································································（5）1.2、主要仪器及试剂···············································································（5）1.3、主要溶液及其配制············································································（5）2、实验方法··························································································（6）2.1、采用Meta-analysis方法分析中国人群p16、RASSF1A基因甲基化与肺癌易感性关系········································································································（6）2.2、被动吸烟对小鼠肺组织中p16、RASSF1A基因甲基化影响实验·················（8）2.3、焦磷酸测序法检测被动吸烟对小鼠p16、RASSF1A基因甲基化的影响程度（10）3、结果······························································································（13）3.1、p16、RASSF1A基因甲基化与中国人群肺癌发生关系的Meta分析···········（13）3.2、被动吸烟对小鼠p16、RASSF1A基因甲基化的影响程度························（17）4、讨论······························································································（21）5、结论······························································································（22）参考文献····························································································（22）文献综述····························································································（27）1、p16基因概述···················································································（28）2、RASSF1A基因概述··········································································（29）3、p16、RASSF1A基因甲基化与肿瘤关系的研究现状···············