信号转导及转录活化因子5对肺癌诊断价值的实验分析-experimental analysis of the diagnostic value of signal transduction and transcription activation factor 5 in lung cancer.docxVIP

下载本文档

4
0
约4.23万字
约 71页
2018-06-03 发布于上海
举报
版权申诉

信号转导及转录活化因子5对肺癌诊断价值的实验分析-experimental analysis of the diagnostic value of signal transduction and transcription activation factor 5 in lung cancer.docx

1、本文档共71页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

信号转导及转录活化因子5对肺癌诊断价值的实验分析-experimental analysis of the diagnostic value of signal transduction and transcription activation factor 5 in lung cancer

PAGE PAGE 10 A549细胞中的表达。（2）STAT5与目前临床上常使用的肺癌标志物 CEA、Cyfra21-1相比较，STAT5对早期肺癌诊断价值相等。（3）STAT5、 CEA、Cyfra21-1三项标志物联合检测能显著提高对肺癌的诊断准确性。因此我们推断STAT5可以作为一种新的肺癌标志物，用与现用的标志物联合检测的方式对肺癌早期诊断具有较高的诊断价值，并推荐临床进一步研究。关键词：肺癌；STAT5；CEA；Cyfra21-1；肺癌标志物 Primary exploration of diagnostic value of STAT5 of lung cancer Abstract Objective:To investigate the expression of STAT5(signal transducer and activator of transcription 5,CEA(carcinoembryonic antig- en) and Cyfra21-1(cytokeratin fragment 19) in normal human embryonic lung cells MRC-5 and lung adenocarcinoma cell A549 .The ROC curve was used to explore the value of STAT5 on diagnosis of lung cancer and the possibility to be a new tumor marker. Methods: 1. Expression of protein of STAT5,CEA and Cyfra21-1 in normal human embryonic lung cells MRC-5 and lung adenocarcinoma cell A549 were tested by immunohistochemistry staining (SP). The pictures were analyzed by Image-Pro Plus version 6.0 (IPP 6.0) professional image analysis software .The degree of stain was determined by mean optical density(MOD) of sections.2.Using realtime fluores-cence quantitative PCR detects STAT5 mRNA,CEA mRNA,Cyfra21-1 mRNA in human lung adenocarcinoma cell line A549 and normal human embryonic lung cells MRC-5.ROC curves analy STAT5, CEA, Cyfra21-1 protein and mRNA expression in human lung adenocarcinoma cell line A549 and normal human embryonic lung cells MRC-5.Results: 1.The results of the immunohistochemistry ： (1)The protein levels of STAT5,CEA and Cyfra21-1 in lung adenocarcinoma cell A549 were apparently higher than that in normal human embryonic lung cells MRC-5.After statistical analysis, they had statistically significant difference(P0.01).(2)STAT5,CEA,Cyfra21-1 andβ-actin were amplified respectively.The amplification efficiency were 97.96%、96.77%、95.19%、 97.73%.(3)The mRNA levels of STAT5 CEA Cyfra21-1 in lung adenocarcinoma cell A549 were higher than normal human embryonic lung cells MRC-5.(4)The area under ROC curves of STAT5 protein an