新型生物印迹脂肪酶及其在1-苯乙醇拆分中应用-novel western blot lipase and its application in resolution of 1 - phenylethanol.docxVIP

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新型生物印迹脂肪酶及其在1-苯乙醇拆分中应用-novel western blot lipase and its application in resolution of 1 - phenylethanol.docx

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新型生物印迹脂肪酶及其在1-苯乙醇拆分中应用-novel western blot lipase and its application in resolution of 1 - phenylethanol

of mass concentration of chitosan to TPP was 1:1 and the volume ratio was 1:1.5; the best cross-linking time was 10min and the temperature was 15oC. The results showed that the chitosan-TPP coated bio-imprinted lipase was spherical and the diameter was about 50nm. Besides, the secondary structure of chitosan-TPP coated lipase was changed obviously.The preparation and characterization of bio-imprinted lipase nanogel: Combining the nanogel with bio-imprinting, the bio-imprinted lipase nanogel was obtained. The optimal preparation conditions were as follows: The optimal concentration of NAS was 7.5mg/ml and the pH was 8. The bio-imprinted lipase nanogel was spherical regularly as chitosan coated lipase and the diameter was about 30~40nm. In addition, most of the bio-imprinted lipase nanogel was distributed singly.KEY WORDS ： bio-imprinted enzyme, bio-imprinted enzyme nanocapsule, bio-imprinted enzyme nanogel, lipase, 1-phenylethanol, chitosan, enantiomeric resolution目录前言.................................................................................................................1第一章文献综述...................................................................................................11.1 分子印迹与生物印迹酶..........................................................................11.1.1 分子印迹原理及制备方法...........................................................11.1.2 分子印迹技术的应用领域...........................................................31.1.3 生物印迹酶原理及制备方法.......................................................41.1.4 影响生物印迹酶的因素...............................................................41.1.5 生物印迹酶的应用.......................................................................51.2 生物催化制备手性化合物研究进展......................................................61.2.1 手性化合物概述...........................................................................61.2.2 生物催化在手性化合物拆分中的应用.......................................71.2.3 脂肪酶不对称拆分 1-苯乙醇 ......................................................71.