一种防治茶小绿叶蝉虫生真菌制剂研制-development of a preparation for prevent and treating insect cicada fungus of tea green leaf.docxVIP

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一种防治茶小绿叶蝉虫生真菌制剂研制-development of a preparation for prevent and treating insect cicada fungus of tea green leaf.docx

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一种防治茶小绿叶蝉虫生真菌制剂研制-development of a preparation for prevent and treating insect cicada fungus of tea green leaf

II II Abstract Abstract: The lesser tea leafhopper, Empoasca vitis (Homoptera: Cicadellidae) is the most serious tea tree pest, which has developed high resistance to almost all insecticides and needs biological alternatives. In the present study, an isolate of Isaria javanicus from the lesser tea leafhopper, Empoasca vitis and 4 isolates of Beauveria bassiana from other leafhoppers were tested for their extracellular protease. I. javanicus isolate and 2 Beauveria isolates were screened out for bioassay to early instar nymphs of the leafhopper. The result indicate that the virulence of I. javanicus isolate was 2.33~7.26 times and 6.08~27.26 times higher than that of two Beauveria isolates, with a LT50 of 5.9 d at the concentration of 1×108 conidia.mL-1, which caused 100% mortality on the 13th d. IJOD-1, an oil dispersion formulation IJOD-1, was developed from the Isaria isolate for field trail. Field trail with randomized block design demonstrated that IJOD-1 sprayed at a dose of 7.5×1013 conidia?ha-1 resulted in population decrease of 50.32% on the 11th d. A supplementary spray was carried out on the 25th d and caused a total population decrease of 68.4% on the 55th d. The storage results of IJOD-1 showed that spore’s germination rate declined slowly both at room temperature and at 4 ℃ within the 1st year of storage, while the lower temperature benefited the storage. Germination rate of spores of stored at 4 ℃ remained 57% and 40.33% on the 12th and the 15th month storage, respectively.Compered with those refrigerated at 4 ℃, spores at room temperature remained a germination rate of 71.33% in 8 months storage. The decrease of germination accelerated in 10 months, and declined to 42.67% and 10.67% on the 12th and 15th month, respectively. However, sorage of pure spores at room temperature decreased germination rate to 61% in 3 months, then germination rate declined sharply and droped to 6.33% in 6 months, and finally no germination was found in 8th