核磁共振内部构造.pptx

Copyright issuesYou can download and use this PowerPoint presentation as you wish, provided the file/presentation is not altered in any wayproper credit is given regarding the origin of the fileDesigned for 24” 1920 x 1200 (16:10) LCD monitorCreated with PowerPoint 2003Superconducting 200 MHzNMR MagnetThe hidden world of Nuclear Magnet Resonance spectroscopyThis slide presentation is dedicated to the memory ofGlen Bigam1939 – 2009who worked in the NMR labs at the Department of Chemistry fornearly 50 years.The picture shows Glen preparing this verymagnet for decommissioning in 2003.This presentation is 15 minutes long and restarts automaticallyContentsWhat is NMR, what can it do? Superconducting magnets: NMR’s heart soulSucrose as an exampleDissection of a magnetChemistryChemistryMedicineIs NMR important?3 Nobel prizes suggest it is:1991 R.R. Ernst Development of the methodology of high-resolution NMR spectroscopy2002 K. Wüthrich Development of NMR spectros-copy for determining the 3-dimensional structure of biological macromolecules in solution 2003 P.C. Lauterbur Sir P. Mansfield Discoveries concerning magnetic resonance imaging (MRI)2003: USA UK1991 2002: SwitzerlandNMR comes in 4 different “flavors”samples in solutionHigh-resolutionSolid-statesamples in solid formMagnetic Resonance Imaging (MRI)pictures of tissue (common diagnostic tool in medicine)In vivo NMR spectroscopyspectra of molecules in human or animal tissueThis magnet was used for solution NMRWhat solution-state NMR can doStructure elucidation of unknown compounds (e.g. natural products isolated from plants)Confirm the structure of synthetic compoundsDetermine the conformation (= 3-dimensional shape) of molecules in solutionStudy interactions of small molecules (drugs) with much larger molecules (proteins, viruses etc.)What nuclei can be measured by NMR?Most elements in the Periodic Table have at least one isotope that is NMR activenatural abundanceH1~100%C1299%C131%N150.4%F19100%P31100% isotopes