一次大强度耐力运动对大鼠骨骼肌蛋白质降解和AMPK活性变化影响.docVIP

一次大强度耐力运动对大鼠骨骼肌蛋白质降解和AMPK活性变化影响 　　摘要：为探讨一次大强度耐力运动过程中，AMPK活性变化对骨骼肌蛋白质降解的作用。将36只 SD大鼠进行一次跑台运动，坡度5%，运动强度25 m/min，运动时间60 min。取样为运动前、运动0.5、1 h，运动后1、2、6 h等6个点。使用高效液相色谱法测定AMP、ATP质量摩尔浓度；采用同位素技术测定腓肠肌中AMPK活性的变化；采用荧光定量PCR技术，测定腓肠肌中MuRF1、MAFbx基因表达量的变化。结果发现：(1)运动0.5 h到运动后即刻，AMP质量摩尔浓度及AMP与ATP质量摩尔浓度比值升高(P 　　关键词：运动生物化学；腺苷酸活化蛋白激酶；泛素蛋白连接酶；蛋白质降解；大鼠；腺苷一磷酸 　　中图分类号：G804.7文献标识码：A文章编号：1006-7116(2011)02-0139-06 　　 　　Effects of one time high intensity endurance exercising on the degradation of skeletal muscle protein and changes of AMPK activity in rats 　　MA Yan-chao1，ZHU Rong2，XU Shou-sheng3，WANG Rui-yuan3 　　(1.School of Physical Education，Luoyang normal University，Luoyang 471022，China；2.Wenzhou Medical College，Wenzhou 325035，China；3.Beijing Sport University，Beijing 100084，China) 　　 　　Abstract: The authors probed into functions played by changes of AMPK activity in the degradation of skeleton muscle protein during one time high intensity endurance exercising. Method: let 36 SD rats do a one time treadmill exercise at a slope of 5%, an exercise intensity of 25 m/min, and an exercise time of 60min. Sampling times were before exercising, after 0.5h and 1h of exercising, 1 h, 2 h and 6 h after exercising. The authors measured AMP and ATP molalities by using high efficiency liquid chromatography, changes of AMPK activity in gastrocnemius muscle by using isotope technology, and changes of MuRF1 and MAFbx gene expressions in gastrocnemius muscle by using fluorescence quantification PCR technology. Results: 1) from the moment after 0.5 h of exercising to the movement immediately after exercising, AMP molality and the ratio of AMP molality to ATP molality increased (P 　　Key words: exercise biochemistry；AMPK；MAFbx；MuRF1 mRNA；protein degradation；rat；AMP 　　 　　 　　泛素蛋白酶体系统是一个主要的蛋白质降解系统，对动物的研究逐渐显示：在肌肉萎缩过程中，泛素蛋白酶体系统促使蛋白质降解。Muscle Ring Finger 1(MuRF1)和Atrophy F-box(MAFbx也称为Atrogin-1)均属于泛素蛋白连接酶，是目前发现的与骨骼肌蛋白质分解代谢关系最为密切的泛素蛋白连接酶。有报道在制动、去神经、后肢悬吊、饥饿以及病理情况(糖尿病、肿瘤、脓毒症)下，骨骼肌萎缩的模型中，MuRF1、MAFbx表达量