两个不同厂家试剂盒检测EB病毒EA-IgAVCA-IgA比较.docVIP

两个不同厂家试剂盒检测EB病毒EA-IgAVCA-IgA比较 　　【摘要】 目的 比较分析两个不同厂家试剂检测EB病毒早期抗原IgA抗体(EA-IgA)、衣壳抗原IgA抗体(VCA-IgA)，并做方法学评价。方法 分别用两个不同厂家的ELISA试剂盒检测184例鼻咽癌(NPC)患者及184例正常人的血清EA-IgA和VCA-IgA。结果 与欧蒙公司的试剂盒相比，IBL公司的试剂EA-IgA的阳性符合率为66.07%，阴性符合率为89.45%，总符合率为82.34%，Kappa值为0.57；VCA-IgA的阳性符合率为67.57%，阴性符合率为99.32%，总符合率为80.16%， Kappa值为0.62。结论 两个厂家的试剂盒在分别验证EA-IgA，VCA-IgA时，具有高度一致性。国内IBL公司生产的试剂可替代进口试剂。 　　【关键词】 EB病毒；鼻咽癌；EA-IgA；VCA-IgA 　　 　　Comparison of the two kits from two different manufacturers in detecting EA-IgA and VCA-IgA of Epstein-Barr virus 　　LUO Ling， ZENG Hua， LUO Xiao-hong. The Sun Yat-sen Memorial Hospital of Sun Yat-sen University,GuangZhou 510120,China 　　【Abstract】 Objective To evaluate the two kits from two different manufacturers in detecting EA-IgA and VCA-IgA of Epstein-Barr virus, then provide the methodology evaluation. Methods The serum of EBV-EA-IgA and VCA-IgA in 184 Nasopharyngeal patients and 184 healthy adults were detected use two kits.Results Compared with the kit of EUROIMMUN, for the kits of IBL to detect EA-IgA, the positive coincidence rate was 66.07%, negative coincidence rate was 89.45% and the total coincidence rate was 82.34%, with Kappa value of 0.57; for VCA-IgA, the positive coincidence rate was 67.57%, negative coincidence rate was 99.32% and the total coincidence rate was 80.16%, with Kappa value of 0.62. Conclusion The two kits have high consistence in screening of EA-IgA and VCA-IgA. The IBL kit produced by the domestic may be a substitute for imported reagents. 　　【Key words】 Epstein-Barr virus; Nasopharyngeal cancer; IgA antibodies against the early antigen of Epstein-Barr virus (EA-IgA); IgA antibodies against the viral capsid antigen of Epstein-Barr virus (VCA-IgA) 　　 　　EB病毒(Epstein-Barr)感染与鼻咽癌等人类恶性疾病的发生密切相关，对诊断鼻咽癌有较高的特异性。定性检测血清中EB病毒抗体已成为早期诊断鼻咽癌的有力工具。近年来随着免疫分析技术的发展，国内外已广泛采用ELISA法检测EB病毒抗体用于鼻咽癌的诊断和筛查。本文就??个不同厂家的试剂盒在检测EB病毒EA-IgA、VCA-IgA的临床价值进行比较。 　　1 资料与方法 　　1.1 标本来源 收集本院2010年1月1日至2010年12月31日在门诊和住院部经病理检查确诊为鼻咽癌的患者的血清标本184例，另外本院同期健康体检者的血清标本184例，年龄为25