中医抗老驻颜古方抗小鼠光老化损伤初步研究.docVIP

中医抗老驻颜古方抗小鼠光老化损伤初步研究 　　[摘要]目的:初步研究中医古医籍记载的美容驻颜抗衰古方可能的抗光老化作用。方法:采用UVA/320～400nm+UVB/280～320nm紫外灯模拟日光紫外线组合造模光老化小鼠,选择中药古医籍记载的美容驻颜抗衰古方每天分别灌胃各组小鼠,共12周。生化法检测小鼠受试区皮肤组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH2Px)活性和丙二醛(MDA)、羟脯氨酸(HYP) 含量。结果:接受实验选择的古医籍记载的美容驻颜抗衰古方治疗的小鼠,表现出不同程度的光保护效果。各组与UV损伤组比较:滋阴为主的神仙驻颜方GSH-Px、HYP表达有极显著性差异(P 　　[关键词]光老化;中医古方;抗衰老;实验研究;小鼠 　　[中图分类号]R289.3 [文献标识码]A [文章编号]1008-6455(2010)04-0578-03 　　 　　Experimental study on the anti-senility effects of several Traditional Ancient Decoctions in mice ageing induced by photoage 　　LIU Yuan1, ZHANG Mi-na2 ,WEI Kai-feng2 ,LU Yuan3,LAI Ming-sheg3,LU Ping-cheng2 　　(1.Shenzhen City Fourth Peoples Hospital, Shenzhen 518033, Guangdong, China;2.Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu,China;3.Shenzhen City sixth Peoples Hospital,Shenzhen 518052, Guangdong,China) 　　 　　Abstract:Objective To investigate the anti-senility effects of several classic doctions mentioned in books of Traditional Chinese Medicine (TCM) to senility induced by photoaging.MethodsA mice model exposed under UVA/320～400nm+UVB/280～320nm UV light to simulate sunlight ultraviolet was served. Mice were feed with several chosen traditional ancient decoctions which were said to have the effects of anti-senility.The experimental procedure lasted for twelve weeks. The levels of superoxide dismutase(SOD), glutathiane peroxidase (GSH-Px), malondialdehyde (MDA) and hydroxyproline(HYP)in the observed skin area of mice before and after experiment were assayed by biochemic methods. Results Mice received treatment of TCM decoctions showed different good effects of anti-photoage. Compared with UV group, Shenxianzhuyan Decoction which mainly menifested enhancing yinshowed higher levels of GSH-Px and Hyp (P 　　 　　紫外线(ultraviolet, UV)慢性损伤导致的皮肤光老化(photoaging)是皮肤提早衰老的重要因素,如何防治光老化已引起美容医学界越来越多的重视。而研究植物草药预防皮肤衰老一直是国内外美容医学界被广泛关注和经常使用的手段,并随着研究的深入和有效的运用,已得到了众多学者们的认同[1-2]。我国运用草药美容驻颜有数千年历史,历代的本草医药著作中收录记载了大量有效美容驻颜的植物,尤其是具有中医医学背景,独特的复方中药。大量研究显示许多中