蛋白制备和纯化.pdf

NIH Public Access Author Manuscript Nat Methods . Author manuscript; available in PMC 2011 September 22. N Published in final edited form as: I H Nat Methods . 2008 February ; 5(2): 135–146. doi:10.1038/nmeth.f.202. - P A A u Protein production and purification t h o r 1,2,3 Structural Genomics Consortium , Architecture et Fonction des Macromolécules M 4 5 a Biologiques , Berkeley Structural Genomics Center , China Structural Genomics n 6,7 8 Consortium , Integrated Center for Structure and Function Innovation , Israel Structural u s 9 10,11 Proteomics Center , Joint Center for Structural Genomics , Midwest Center for c r 12 i Structural Genomics , New York Structural GenomiX Research Center for Structural p t Genomics13,14,15,16,17, Northeast Structural Genomics Consortium18,19, Oxford Protein Production Facility20, Protein Sample Production Facility, Max Delbrück Center for Molecular Medicine21, RIKEN Structural Genomics/Proteomics Initiative22, and SPINE2- Complexes23,25 1Karolinska Institutet, Schéeles väg 2, 171 77 Stockholm, Sweden. 2University of Oxford, Old Road Campus, Roosevelt Drive, Headington, Oxford OX3 7DQ, UK. N I 3 H University of Toronto, 100 College St., Toro